Kv2.1 K〔+〕 Channels Underlie Major Voltage-Gated K〔+〕 Outward Current in H9c2 Myoblasts
-
- Wang Wei
- Department of Physiology and Department of Parasitology, Juntendo University School of Medicine
書誌事項
- タイトル別名
-
- KV2.1 K+ Channels Underlie Major Voltage-Gated K+ Outward Current in H9c2 Myoblasts.
この論文をさがす
説明
The H9c2 clonal cell line derived from embryonic rat ventricle is an in vitro model system for cardiac and skeletal myocytes. We used the whole-cell patch clamp technique to characterize the electrophysiological and pharmacological properties of an outward K+ current (IKV) and determined its molecular correlate in H9c2 myoblasts. IKV was activated by threshold depolarization to −30 mV, and its current amplitude and rate of activation increased with further depolarizations. IKV inactivated slowly with a time constant of 1–2 s, and the V0.5 for steady-state inactivation was −37.9 ± 4.6 mV (n = 10). Tetraethylammonium and quinidine suppressed IKV with IC50's of 3.7 mM and 11.6 μM, respectively. Using RT-PCR analysis we found that the KV 2.1 gene is the most abundantly expressed among genes for KV1.2, 1.4, 1.5, 2.1, 4.2, and 4.3, and by Western blotting we confirmed the synthesis of the KV2.1 α-subunit protein. We conclude that IKV, the predominant voltage-gated outward current in H9c2 myoblasts, flows through the channels comprised of the KV2.1-subunit gene product.<br>
収録刊行物
-
- The Japanese Journal of Physiology
-
The Japanese Journal of Physiology 52 (6), 507-514, 2002
一般社団法人 日本生理学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282680019633536
-
- NII論文ID
- 50000764303
- 130004435849
-
- NII書誌ID
- AA00691224
-
- COI
- 1:CAS:528:DC%2BD3sXis1Oku7g%3D
-
- ISSN
- 18811396
- 0021521X
-
- NDL書誌ID
- 6465716
-
- PubMed
- 12617756
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可