Generation of a Polymorphic Marker Linked to Thymoma Susceptibility Gene of Rat 1 by Genetically-Directed Representational Difference Analysis.

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  • HIGO Kyoko
    Department of Pathology, Fujita Health University School of Medicine
  • USHIJIMA Toshikazu
    Carcinogenesis Division, National Cancer Center Research Institute
  • OYABU Atsushi
    Department of Internal Medicine, Daiko Medical Center, Nagoya University School of Medicine
  • YE Chunlin
    Department of Pathology, Fujita Health University School of Medicine
  • YAGYU Shigeru
    Institute for Comprehensive Medical Sciences, Fujita Health University School of Medicine
  • TAKAHASHI Hisahide
    Institute for Comprehensive Medical Sciences, Fujita Health University School of Medicine
  • MATSUYAMA Mutsushi
    Department of Pathology, Fujita Health University School of Medicine

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抄録

BUF/Mna (BUF) is a rat strain susceptible to spontaneous development of thymomas. We have previously shown that the thymoma susceptibility is controlled principally by a dominant susceptibility gene located on chromosome 7, thymoma susceptibility gene of rat 1 (Tsr1). To generate genetic markers tightly linked to Tsr1, we performed genetically directed representational difference analysis (GDRDA) with three combinations of the tester and driver DNAs. From 124 {ACI/NMs × (BUF × ACI/NMs) F1} backcross rats, 12 rats with the ACI/BUF genotype in the Tsr1 region (A/B rats) and 13 rats with the ACI/ACI genotype in the region (A/A rats) were selected, and their DNAs were pooled, respectively. Three kinds of tester DNAs, i) inbred BUF, ii) (BUF × ACI)F1, and iii) the pool from the A/B rats, were subtracted by the driver DNA prepared from the pool of the A/A rats. The three combinations yielded one, two, and one polymorphic marker(s), respectively. One marker, D7Ncc28, was isolated commonly by the three combinations of subtraction, and another marker, D11Ncc12 was isolated only by the second combination. Linkage analysis demonstrated that D7Ncc28 was located in the 8.3 cM region where Tsr1 has been mapped. The three combinations of subtraction were shown to be almost equally capable of isolating polymorphic markers in a specific chromosomal region.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 49 (3), 189-195, 2000

    公益社団法人 日本実験動物学会

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