Direct Experimental Occlusion of the Distal Middle Cerebral Artery Induces High Reproducibility of Brain Ischemia in Mice
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- KURAOKA Mutsuki
- Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo
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- FURUTA Takahisa
- Department of Microbiology and Immunology, The Institute of Medical Science, University of Tokyo
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- MATSUWAKI Takashi
- Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, University of Tokyo
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- OMATSU Tsutomu
- Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo
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- ISHII Yoshiyuki
- Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo
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- KYUWA Shigeru
- Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo
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- YOSHIKAWA Yasuhiro
- Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo
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抄録
Several investigators have used murine models to investigate the pathophysiology of brain ischemia. The focal ischemic model is a closer approximation to human stroke which includes a necrotic core, penumbra, and undamaged tissue. Occlusion of a unilateral artery, especially the middle cerebral artery (MCA), is performed in this model, but collateral circulation often induces variation of ischemic lesions both qualitatively and quantitatively. It is likely that the more proximal the artery which is unilaterally occluded is, the more inconsistent the outcomes. The present study was designed to examine the reproducibility of infarct lesion by distal or proximal artery occlusion. Direct occlusion of the distal MCA was performed and compared with unilateral common carotid artery occlusion (CCAO) in C57BL/6 mice. Direct MCA occlusion (MCAO) consistently induced ischemic lesions in cortical areas. All model animals (n=14) survived 24 h after occlusion, and exhibited a maximum infarct volume (20.0 ± 5.0%). In contrast, permanent and transient unilateral CCAO models had mortality rates of 62.5 and 25.0%, and showed severe to absent lesions with the infarct volumes of 29.0 ± 20.8 and 33.2 ± 24.2%, respectively. In conclusion, distal MCAO produces high reproducibility of ischemic insults and survivability compared to unilateral CCAO. Thus, distal MCAO is a useful method for the focal ischemic model.<br>
収録刊行物
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- Experimental Animals
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Experimental Animals 58 (1), 19-29, 2009
公益社団法人 日本実験動物学会
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詳細情報 詳細情報について
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- CRID
- 1390282680021161728
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- NII論文ID
- 130000419480
- 10024837886
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- NII書誌ID
- AA11032321
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- ISSN
- 18817122
- 00075124
- 13411357
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- NDL書誌ID
- 9760744
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可