Evaluation of a New Enzyme Immunoassay (TESTPACK ROTAVIRUS<SUP>®</SUP>) for Diagnosis of Viral Gastroenteritis
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- HONMA Hitoshi
- Division of Special Pathogens, Department of Enteroviruses, National Institute of Health Department of Bacteriology, The Jikei University School of Medicine
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- USHIJIMA Hiroshi
- Division of Special Pathogens, Department of Enteroviruses, National Institute of Health
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- TAKAGI Michio
- Department of Pediatrics, Maizuru Kyosai Hospital
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- KITAMURA Takashi
- Division of Special Pathogens, Department of Enteroviruses, National Institute of Health
Bibliographic Information
- Other Title
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- ウイルス性胃腸炎の診断のための新しい酵素抗体法 (テストパツク・ロタウイルス<SUP>®</SUP>) の評価
- Evaluation of a New Enzyme Immunoassay (TESTPACK ROTAVIRUS^|^reg;) for Diagnosis of Viral Gastroenteritis
Abstract
TESTPACK ROTAVIRUS®, a simple 10 min enzyme immunoassay, was compared with reversed passive hemagglutination assay (RPHA) and polyacrylamide gel electrophoresis of virus RNA (RNA-PAGE) for the detection of rotaviral antigens in feces from 104 diarrheal children. The reults of TESTPACK ROTAVIRUS® were identical with those of RNA-PAGE. Discordant results of RPHA were further examined by latex agglutination and electron microscopy, and were finally decided to be negative. Pararotaviruses, adenoviruses and small round viruses were negative in the TESTPACK ROTAVIRUS®.
Journal
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- Kansenshogaku Zasshi
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Kansenshogaku Zasshi 64 (2), 174-178, 1990
The Japanese Association for Infectious Diseases