Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo
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- HASUWA Hidetoshi
- Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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- MURO Yuko
- Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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- IKAWA Masahito
- Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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- KATO Noriko
- Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School JCL Bioassay Corp.
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- TSUJIMOTO Yoshihide
- Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School
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- OKABE Masaru
- Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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Abstract
In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.<br>
Journal
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- Experimental Animals
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Experimental Animals 59 (1), 105-107, 2010
Japanese Association for Laboratory Animal Science
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Details 詳細情報について
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- CRID
- 1390282680021416064
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- NII Article ID
- 10027852768
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- NII Book ID
- AA11032321
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- COI
- 1:CAS:528:DC%2BC3cXkvFGrtL0%3D
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- ISSN
- 18817122
- 00075124
- 13411357
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- NDL BIB ID
- 10811120
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- PubMed
- 20224175
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed