The status of donor cancer tissues affects the fate of patient-derived colorectal cancer xenografts in NOG mice
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- FUJII Etsuko
- Research Division, Chugai Pharmaceutical Co., Ltd., 200 Kajiwara, Kamakura, Kanagawa 247-8530, Japan
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- KATO Atsuhiko
- Research Division, Chugai Pharmaceutical Co., Ltd., 200 Kajiwara, Kamakura, Kanagawa 247-8530, Japan
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- CHEN Yu Jau
- Chugai Pharmabody Research, Pte. Ltd., 3 Biopolis Drive, #04-11 Synapse, Singapore 138623
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- MATSUBARA Koichi
- Chugai Pharmabody Research, Pte. Ltd., 3 Biopolis Drive, #04-11 Synapse, Singapore 138623 PharmaLogicals Research, Pte. Ltd., 11 Biopolis Way, #05-08/09 Helios, Singapore 138667
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- OHNISHI Yasuyuki
- Central Institute for Experimental Animals, 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821 Japan
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- SUZUKI Masami
- Research Division, Chugai Pharmaceutical Co., Ltd., 200 Kajiwara, Kamakura, Kanagawa 247-8530, Japan
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抄録
Patient-derived xenografts (PDXs) of tumors are increasingly becoming important tools for translational research in oncology. The NOD.Cg-Prkdcscid Il2rgtm1Sug/Jic (NOG) mouse is an efficient host for PDXs. Thus as a basis for future development of methods to obtain PDXs from various disease types, we have studied the factors that affect the outcome of transplantation of human colorectal cancer in NOG mice. Of the original donor cases examined, 73% had successful engraftment. The outcome of donor-matched tissues was consistent in most cases, and was thought to show that the condition of the host did not affect engraftment. Next we analyzed the tumor aggressiveness in terms of histology grade of the original tumor and found that they were related to engraftment. Detailed histopathological examination of the transplanted tissues strongly indicated that lymphocytes engrafted with the tumor cells affect engraftment. As a factor related to transplantation of lymphocytes, we studied the human IgG concentration in the serum of tumor-bearing mice, but there was no tendency for higher concentrations to result in unsuccessful engraftment. Finally, we studied the type, density and location of T cells in the original donor tissue to determine the immune contexture and found that the unsuccessful engraftment cases tended to have an adequate or coordinated immune contexture compared to successful engraftment cases. From these results, we concluded that the aggressiveness and the T cell infiltration of the original tumor affect the outcome of transplantation in the NOG mouse.
収録刊行物
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- Experimental Animals
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Experimental Animals 64 (2), 181-190, 2015
公益社団法人 日本実験動物学会
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詳細情報 詳細情報について
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- CRID
- 1390282680021651072
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- NII論文ID
- 130005069863
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- NII書誌ID
- AA11032321
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- ISSN
- 18817122
- 00075124
- 13411357
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- NDL書誌ID
- 026335858
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- PubMed
- 25740629
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可