Evaluation of Gelatin Particle Agglutination Method for Detection of <I>Treponema pallidum</I> Antibody

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  • DEGUCHI Matsuo
    The Laboratory for Clinical Investigation, Osaka University Hospital
  • HOSOTSUBO Hideo
    The Laboratory for Clinical Investigation, Osaka University Hospital
  • YAMASHITA Naoko
    The Laboratory for Clinical Investigation, Osaka University Hospital
  • OHMINE Toshinari
    The Laboratory for Clinical Investigation, Osaka University Hospital
  • ASARI Seishi
    The Laboratory for Clinical Investigation, Osaka University Hospital

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Other Title
  • 梅毒<I>Treponema pallidum</I>抗体測定におけるゼラチン粒子凝集法の評価
  • 梅毒Treponema pallidum抗体測定におけるゼラチン粒子凝集法の評価
  • バイドク Treponema pallidum コウタイ ソクテイ ニ オケル
  • Evaluation of Gelatin Particle Agglutination Method for Detection of Treponema pallidum Antibody

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Abstract

Treponema pallidum hemagglutination (HA) is one of the most frequently used methods for the detection of Treponema pallidum (T. pallidum) antibodies. Recently, an innovative agglutination method using artificial carriers was newly developed, and is now available as a routine method.<BR>In order to compare the newly developed particle agglutination (PA) method (FUJIREBIO INC.) with the conventional HA method, T pallidum antibody titers of numerous sera were measured by respective methods.<BR>In the stability study, reconstituted reagent was stable for at least three weeks. Sample inactivation (56°C/30 min) demonstrated no effect on the test results.<BR>Among 800 sera, 132 (16.6%) positives (+), 633 (79.1%) negatives (-) and 34 (4.3%) indeterminates (+) were obtained by HA method. Meanwhile, 144 (18.0%) positives (+), 627 (78.4%) negatives (-) and 29 (3.6%) indeterminates (+) were obtained by PA method. The correlation between PA and HA method was 97.8%, and the antibody titers obtained by PA method showed good correlation with HA method.<BR>Those samples which showed discrepancy between PA and HA method in the above study were further examined with fluorescent treponemal antibody-absorption (FTA-ABS) method. The results obtained from FTA-ABS method were almost consistent with those obtained from PA method.<BR>For respective syphilis patients in stage I and II, antibody titer was monitored by HA, PA and RPR method. The results indicated that changes in antibody titer obtained from PA method was approximately the same as the titer changes obtained from RPR method. Namely, PA method detected the presence of IgM earlier than HA method. Further, changes in antibody level accompanied with the disease treatment were detected by PA method, and the changes in antibody titer correspond to the clinical progress of the disease.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 68 (10), 1271-1277, 1994

    The Japanese Association for Infectious Diseases

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