Detection of the Thermostable Lipase Gene (<I>lipAH</I>) in Clinical Isolates of <I>Aeromonas hydrophila</I> Belonging to the Major 0 Serogroups 011, 016 and 034

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Other Title
  • <I>Aeromonas hydrophila</I> の主要O群株からの耐熱性リパーゼ遺伝子の検出
  • Aeromonas hydrophilaの主要O群株からの耐熱性リパーゼ遺伝子の検出
  • Aeromonas hydrophila ノ シュヨウ Oグン カブ カラ ノ
  • Detection of the Thermostable Lipase Gene (lipAH) in Clinical Isolates of Aeromonas hydrophila Belonging to the Major 0 Serogroups 011, 016 and 034

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Abstract

On the basis of DNA hybridization data and phenotypes, most pathogenic strains of Aerornonas hydrophila are grouped into hybridization group 1 (HG1). These pathogenic strains secret the theromostable lipase, and it's gene (lipAH) has been cloned and sequenced. The present study was performed to identify the pathogenic strains of A. hydrophila by the polymerase chain reaction (PCR) technique to detect the lipAH. Synthetic oligonucleotide primers (lipAH-ns-1 and-na-1) were used in the PCR. The PCR identified 80% of lipAH-positive strains, consisting of seven of the 11 011 strains (64%), 13 of the 15 016 strains (87%) and 25 of the 30 034 strains (83%) in clinical isolates of A. hydrophila used in this study.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 71 (11), 1172-1174, 1997

    The Japanese Association for Infectious Diseases

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