Rapid Detection of the Hemolysin Genes in <I>Aeromonas sobria</I> by the Polymerase Chain Reaction
-
- SHIBATA Mikiyoshi
- Department of Microbiology, Kyorin University School of Health Sciences
-
- MORITA Koji
- Department of Microbiology, Kyorin University School of Health Sciences
-
- WATANABE Noboru
- Department of Microbiology, Kyorin University School of Health Sciences
-
- WADA Hiroshi
- Department of Microbiology, Kyorin University School of Health Sciences
-
- OKITSU Tadayuki
- Kanagawa Prefectual Public Health Laboratory
-
- YAMAI Shiro
- Kanagawa Prefectual Public Health Laboratory
-
- ITOH Ken-ichiro
- Department of Bacteriology, National Institute of Health
-
- SHIMADA Toshio
- Department of Bacteriology, National Institute of Health
-
- WATANABE Haruo
- Department of Bacteriology, National Institute of Health
-
- KANAMORI Masato
- Department of Microbiology, Kyorin University School of Health Sciences
Bibliographic Information
- Other Title
-
- PCR法による<I>Aeromonas sobria</I>のヘモリシン遺伝子の検出
- PCR法によるAeromonas sobriaのヘモリシン遺伝子の検出
- PCRホウ ニヨル Aeromonas sobria ノ ヘモリシン イデンシ
- Rapid Detection of the Hemolysin Genes in Aeromonas sobria by the Polymerase Chain Reaction
Search this article
Abstract
The hemolysin of Aeromonas sobria is one of the important virulence factors in this organism. Rapid detection and identification test for A. sobria is important for early and specific diagnosis of this infectious disease. We evaluated the Polymerase chain reaction (PCR) for the rapid detection of A. sobria. Two pairs of synthetic oligonucleotide primers (ASA1-s and a; AerAAS-s and a) were used in PCR technique to detect the different hemolysin genes (ASA1 and aerAAS) in A. sobria. The PCR identified 91% of ASAl-positive and 23.4% of aerAAS-positive strains in β-hemolytic A. sobria. Other species of Aeromonas, Plesiomonas shigelloides, Vibrio cholerae 01 and V. parahaemolyticus tested were negative in the PCR with two pairs of primers. The PCR technique for detection of two hemolysin genes suggested the possibility of application of this method for detection of A. sobria in A. sobria-associated infections.
Journal
-
- Kansenshogaku Zasshi
-
Kansenshogaku Zasshi 70 (12), 1266-1270, 1996
The Japanese Association for Infectious Diseases
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282680023299200
-
- NII Article ID
- 130004330442
- 10008724328
-
- NII Book ID
- AN00047715
-
- ISSN
- 1884569X
- 03875911
-
- NDL BIB ID
- 4108000
-
- PubMed
- 9011120
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed