Use of Caco-2 Cells for Isolation of Influenza Virus
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- YOSHINO Syuji
- Miyazaki Prefectural Institute for Public Health and Environment
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- YAMAMOTO Seigo
- Miyazaki Prefectural Institute for Public Health and Environment
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- KAWABATA Norihiko
- Miyazaki Prefectural Institute for Public Health and Environment
Bibliographic Information
- Other Title
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- Caco-2細胞を用いたインフルエンザウイルスの分離
- Caco-2 サイボウ オ モチイタ インフルエンザ ウイルス ノ ブンリ
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Abstract
In this study we assessed the usefulness of Caco-2 cells, derived from a human colon carcinoma, to isolate an influenza virus.<BR>Throat washings collected from 30 patients with influenza-like illnesses in Miyazaki Prefecture in 1997 were inoculated in MDCK and Caco-2 cells, 17 influenza virus strains were isolated in MDCK cells, and 20 in Caco-2 cells. Of all the viruses isolated, only one strain was identified as influenza virus type B; other strains were identified as type A (H3N2). Furthermore, some influenza viruses were isolated in Caco-2 cells also from the specimens collected between 1991 and 1997. With Caco-2 cells, each type of influenza virus was isolated effectively without the supplement of trypsin in the culture medium.<BR>These facts indicate the usefulness of Caco-2 cells as a host to isolate influenza virus as shown to be suitable in the detection of many types of enteric viruses. Caco-2 cells will serve as a useful cell line for the surveillance of infectious disease because Caco-2 cells are sensitive to a wide range of virus.
Journal
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- Kansenshogaku Zasshi
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Kansenshogaku Zasshi 72 (4), 347-351, 1998
The Japanese Association for Infectious Diseases
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Keywords
Details 詳細情報について
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- CRID
- 1390282680023349120
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- NII Article ID
- 130004330671
- 10008717747
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- NII Book ID
- AN00047715
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- ISSN
- 1884569X
- 03875911
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- NDL BIB ID
- 4482704
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- PubMed
- 9621562
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed