Immunological Specificity of Helicobacter pylori Urease and Identification by Immunological Detection of Its Specific Urease

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  • SHINGAKI Masao
    The Tokyo Metropolitan Research Laboratory of Public Health
  • KAI Akemi
    The Tokyo Metropolitan Research Laboratory of Public Health
  • ITOH Takeshi
    The Tokyo Metropolitan Research Laboratory of Public Health
  • HIRATA Ichiro
    Tama Branch Laboratory, The Tokyo Metropolitan Research Laboratory of Public Health

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Other Title
  • <I>Helicobacter pylori</I>が産生するウレアーゼの免疫学的特異性とラテックス凝集反応による本菌の同定
  • Helicobacter pyloriが産生するウレアーゼの免疫学的特異性とラテックス凝集反応による本菌の同定〔英文〕
  • Helicobacter pylori ガ サンセイスル ウレアーゼ ノ メン

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Abstract

Helicobacterpylori urease was recovered as a single peak by DEAE-Sepharose column chromatography and Sephacryl S-200 gel filtration. The purified urease was obtained by fast protein liquid chromatography using a Mono Q column. The purified urease preparation gave a single band in polyacrylamide gel discelectrophoresis.<BR>Latex particles were sensitized with anti-urease immunoglobulin. The sensitized latex particles were agglutinated with the purified urease and by cell sonicates obtained from 55 strains of H. pylori which were isolated from the gastric mucosa of patients with gastric and duodenal disorders, while they did not react with those obtained from related bacteria known to be urease producers, such as Helicobacter mustelae and urease-positive “Campylobacter lari variants”, or by urease of some strains of Enterobacteriae. We have developed a specific and sensitive method for detecting the urease by using the reversed passive latex agglutination technique, in order to identify of the organism.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 67 (11), 1076-1082, 1993

    The Japanese Association for Infectious Diseases

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