Study of <I>Legionella pneumophila</I> Detection by Two Step Polymerase Chain Reaction

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  • KOIDE Michio
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus
  • SAITO Atsushi
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus
  • HIGA Futoshi
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
  • YAMASHIRO Yuuko
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
  • ISHIMINE Tomohiko
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
  • FUTENMA Mitsuhiko
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
  • INADOME Jun
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
  • KAWAKAMI Kazuyoshi
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
  • KUSANO Nobuchika
    First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus

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Other Title
  • Two Step Polymerase Chain Reaction法による<I>Legionella pneumophila</I>の検出
  • Two Step Polymerase Chain Reaction法によるLegionella pneumophilaの検出
  • Two Step Polymerase Chain Reactionホウ ニ
  • Study of Legionella pneumophila Detection by Two Step Polymerase Chain Reaction

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Abstract

We studied theusefulness of two sets of primers in macrophage infectivity potentiator gene sequence of Legionella pneumophila. PCR by 1st step primers produced 649 bp DNA bands and 2nd step primers 489 bp DNA bands. Two step PCR by these primers produced 489 bp DNA bands specific for L. pneumophila. Two step PCR detected 10 fg of pure DNA extracted from L. pneumophila, Philadelphia-1 strain. PCR sensitivity by these primers was superior to former primers reported by us using intratracheal aspirates collected from the patient with L. pneumophila serogroup 2 pneumonia.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 67 (11), 1062-1067, 1993

    The Japanese Association for Infectious Diseases

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