Re-Establishment of Complement C6-Deficient Rabbit Colony by Cryopreserved Sperm Transported from Abroad

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  • LIU Enqi
    Analytical Research Center for Experimental Sciences, Saga University Laboratory Animal Center, Xi'an Jiaotong University School of Medicine
  • KITAJIMA Shuji
    Analytical Research Center for Experimental Sciences, Saga University
  • WIESE Elena
    Central Laboratory Animal Facility, Johannes Gutenberg-Universität Mainz
  • REIFENBERG Kurt
    Central Laboratory Animal Facility, Johannes Gutenberg-Universität Mainz
  • MORIMOTO Masatoshi
    Analytical Research Center for Experimental Sciences, Saga University
  • WATANABE Teruo
    Analytical Research Center for Experimental Sciences, Saga University
  • FAN Jianglin
    Interdisciplinary Graduate School of Medical and Engineering, University of Yamanashi

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Abstract

Introducing rabbits as genetic materials into institutes for experimental animals from other colonies is essential for biomedical research. Currently, it is inconvenient to transport live rabbits from abroad, since they suffer from stress, are prone to accidents and must be inspected, as well as endure quarantine during the often long journey. To overcome these limitations of live animals, we transported sperm cryopreserved in liquid nitrogen. Rabbit sperm was collected from complement C6-deficient rabbits in Germany and then transported to Japan using a dry-shipper containing liquid nitrogen. After thawing the frozen semen and artificial insemination (AI), eleven live pups were born. Subsequently, a homozygous C6-deficient rabbit colony was established. <br>

Journal

  • Experimental Animals

    Experimental Animals 56 (2), 167-171, 2007

    Japanese Association for Laboratory Animal Science

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