Transcutaneous Cisternal Puncture for Sampling of Cerebrospinal Fluid in Awake Rat

DOI Web Site Web Site PubMed 4 Citations 13 References Open Access
  • TAKASUGI Yoshihiro
    Department of Anesthesiology, Kinki University School of Medicine
  • SHIRAI Toru
    Department of Anesthesiology, Kinki University School of Medicine
  • FUTAGAWA Koichi
    Department of Anesthesiology, Nara Hospital, Kinki University School of Medicine
  • KOGA Yoshihisa
    Department of Anesthesiology, Kinki University School of Medicine
  • EGAWA Kentaro
    Life Science Research Institute, Kinki University School of Medicine
  • WATANABE Shinsuke
    Life Science Research Institute, Kinki University School of Medicine
  • UMEDA Takashi
    Department of Clinical Acupuncture, Osaka Kansai College of Oriental Medicine

Search this article

Description

Reported cisternal puncture methods require the anesthetization and fixation of an animal within a stereotaxic frame. To determine the effect of anesthesia and animal fixation on the central nervous system (CNS), amino acid concentrations of cerebrospinal fluid (CSF) sampled by transcutaneous cisternal puncture were compared among awake rats, pentobarbital-anesthetized rats and pentobarbital-anesthetized rats fixed in a stereotaxic frame. Although the concentrations of many amino acids in the CSF of pentobarbital-anesthetized rats were lower than in awake rats, use of the stereotaxic frame resulted in significantly increased amino acid concentrations in the CSF. These data indicate that CSF sampling by transcutaneous cisternal puncture from awake rats is a suitable method for serial measurement of drug effects on the CNS.<br>

Journal

  • Experimental Animals

    Experimental Animals 54 (2), 193-196, 2005

    Japanese Association for Laboratory Animal Science

Citations (4)*help

See more

References(13)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top