Transcutaneous Cisternal Puncture for Sampling of Cerebrospinal Fluid in Awake Rat

TAKASUGI Yoshihiro, SHIRAI Toru, FUTAGAWA Koichi, KOGA Yoshihisa, EGAWA Kentaro, WATANABE Shinsuke, UMEDA Takashi

doi:10.1538/expanim.54.193

Transcutaneous Cisternal Puncture for Sampling of Cerebrospinal Fluid in Awake Rat

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TAKASUGI Yoshihiro

Department of Anesthesiology, Kinki University School of Medicine
SHIRAI Toru

Department of Anesthesiology, Kinki University School of Medicine
FUTAGAWA Koichi

Department of Anesthesiology, Nara Hospital, Kinki University School of Medicine
KOGA Yoshihisa

Department of Anesthesiology, Kinki University School of Medicine
EGAWA Kentaro

Life Science Research Institute, Kinki University School of Medicine
WATANABE Shinsuke

Life Science Research Institute, Kinki University School of Medicine
UMEDA Takashi

Department of Clinical Acupuncture, Osaka Kansai College of Oriental Medicine

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説明

Reported cisternal puncture methods require the anesthetization and fixation of an animal within a stereotaxic frame. To determine the effect of anesthesia and animal fixation on the central nervous system (CNS), amino acid concentrations of cerebrospinal fluid (CSF) sampled by transcutaneous cisternal puncture were compared among awake rats, pentobarbital-anesthetized rats and pentobarbital-anesthetized rats fixed in a stereotaxic frame. Although the concentrations of many amino acids in the CSF of pentobarbital-anesthetized rats were lower than in awake rats, use of the stereotaxic frame resulted in significantly increased amino acid concentrations in the CSF. These data indicate that CSF sampling by transcutaneous cisternal puncture from awake rats is a suitable method for serial measurement of drug effects on the CNS.<br>