Nasal immunization with the 40-kDa outer membrane protein of Porphyromonas gingivalis plus cholera toxin induces protective immunity in aged mice
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- Cai Yu
- Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
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- Kurita-Ochiai Tomoko
- Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
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- Kobayashi Ryoki
- Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
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- Hashizume Tomomi
- Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
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- Yamamoto Masafumi
- Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
書誌事項
- タイトル別名
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- Nasal immunization with the 40-kDa outer membrane protein of <i>Porphyromonas gingivalis</i> plus cholera toxin induces protective immunity in aged mice
- 公開日
- 2013
- 資源種別
- journal article
- DOI
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- 10.2334/josnusd.55.107
- 公開者
- 日本大学歯学部
この論文をさがす
説明
Previous studies have demonstrated that nasal administration of the outer membrane protein of Porphyromonas gingivalis (40k-OMP) with cholera toxin (CT) as an adjuvant elicits protective immune responses against P. gingivalis in young mice. In the present study, we investigated whether administration of 40k-OMP plus CT would also induce 40k-OMP-specific antibody (Ab) responses to provide protective immunity against P. gingivalis infection in aged mice. Nasal immunization with 40k-OMP plus CT elicited 40k-OMP-specific IgG and IgA Ab responses in serum and a significant anti-40k-OMP IgA Ab response in nasal washes and saliva in 1- and 2-year-old mice. Furthermore, both Th1- and Th2-type cytokine responses were induced by the immunization, and cytokine-associated IgG1, IgG2a, and IgG2b Ab responses were observed in the spleens of aged mice. Although the aged mice showed lower 40k-OMP-specific Ab responses than young mice, their mucosal IgA Ab titers as well as serum IgG Ab responses indicated a retained ability to mediate protective immunity; the only exception was saliva in 2-year-old mice. These findings suggest that nasal immunization with 40k-OMP plus CT can be a potential vaccination strategy for eliciting levels of Abs sufficient to provide protective immunity against P. gingivalis infection in aged mice. (J Oral Sci 55, 107-114, 2013)
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
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Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 55 (2), 107-114, 2013
日本大学歯学部