Gender Difference in the Composition of Fecal Flora in Laboratory Mice, as Detected by Denaturing Gradient Gel Electrophoresis (DGGE)

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  • FUSHUKU Seigo
    Center for Laboratory Animal Science, National Defense Medical College
  • FUKUDA Koichi
    Center for Laboratory Animal Science, National Defense Medical College

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Abstract

The difference between genders in the composition of mouse fecal flora was examined. A polymerase chain reaction followed by denaturing gradient gel electrophoresis (DGGE) were performed on the V6-V8 regions of bacterial 16S rDNA obtained from fecal samples at 0, 1, 2, 3, 4, and 8 weeks after the introduction of mice into the laboratory from a mouse farm. Cluster analysis and non-metric multidimensional scaling (NMDS) were then performed. Male and female mice were distributed on opposite sides of the origin of the plane in NMDS at weeks 0, 2, 3, and 8. These results suggest a gender difference in the composition of intestinal flora in mice.<br>

Journal

  • Experimental Animals

    Experimental Animals 57 (5), 489-493, 2008

    Japanese Association for Laboratory Animal Science

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