Two Outbreaks of <i>Yersinia enterocolitica </i>O：8 Infections in Tokyo and the Characterization of Isolates

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  • KONISHI Noriko
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • ISHITSUKA Rie
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • YOKOYAMA Keiko
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • SAIKI Dai
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • AKASE Satoru
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • MONMA Chie
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • HIRAI Akihiko
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • SADAMASU Kenji
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • KAI Akemi
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health

Bibliographic Information

Other Title
  • 東京都内で発生した<i>Yersinia enterocolitica </i>血清群O8 による 集団下痢症2 事例と分離菌株の細菌学的検討
  • 東京都内で発生したYersinia enterocolitica血清群O8による集団下痢症2事例と分離菌株の細菌学的検討
  • トウキョウ トナイ デ ハッセイ シタ Yersinia enterocolitica ケッセイグン O8 ニ ヨル シュウダン ゲリショウ 2 ジレイ ト ブンリ キンカブ ノ サイキンガクテキ ケントウ

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Description

Although the number of outbreaks caused by Yersinia enterocolitica has been very small in Japan, 4 outbreaks were occurred during the 2 years between 2012 and 2013. We describe herein 2 outbreaks which were examined in Tokyo in the present study. Outbreak 1：A total of 39 people (37 high school students and 2 staff) stayed at a hotel in mountain area in Japan had experienced abdominal pain, diarrhea and fever in August, 2012. The Y. enterocolitica serogroup O：8 was isolated from 18 (64.3％) out of 28 fecal specimens of 28 patients. The infection roots could not be revealed because Y. enterocolitica was not detected from any meals at the hotel or its environment. Outbreak 2：A total of 52 students at a dormitory had diarrhea and fever in April, 2013. The results of the bacteriological and virological examinations of fecal specimens of patients showed that the Y. enterocolitica serogroup O：8 was isolated from 24 fecal specimens of 21 patients and 3 kitchen staff. We performed bacteriological and virological examination of the stored and preserved foods at the kitchen of the dormitory to reveal the suspect food. For the detection of Y. enterocolitica, food samples together with phosphate buffered saline (PBS) were incubated at 4℃for 21 days. Then, a screening test for Y. enterocolitica using realtime-PCR targeting the ail gene was performed against the PBS culture. One sample (fresh vegetable salad) tested was positive on realtime-PCR. No Y. enterocolitica was isolated on CIN agar from the PBS culture because many bacteria colonies other than Y. enterocolitica appeared on the CIN agar. After the alkalinetreatments of the culture broth or the immunomagnetic beads concentration method using anti-Y. enterocolitica O：8 antibodies, Y. enterocolitica O：8 w h ich was the same serogroup as the patientsʼisolates was successfully isolated from the PBS culture. The fresh vegetable salad was confirmed as the incrimination food of this outbreak.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 90 (1), 66-72, 2016

    The Japanese Association for Infectious Diseases

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