Anti-drug Pattern of Drug-resistant <I>Mycobacterium tuberculosis</I> and Analysis of Mutation in Drug-target Genes

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  • MUKAIGAWA Jun
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • ENDOH Miyoko
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • YANAGAWA Yoshitoki
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • MOROZUMI Satoshi
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health

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Other Title
  • 薬剤耐性結核菌株の薬剤耐性パターンと遺伝子変異の解析
  • ヤクザイ タイセイ ケッカク キンカブ ノ ヤクザイ タイセイ パターン ト イデンシ ヘンイ ノ カイセキ
  • Anti-drug Pattern of Drug-resistant Mycobacterium tuberculosis and Analysis of Mutation in Drug-target Genes

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Abstract

The antimycobacterial susceptibility test was performed and minimal inhibitory concentration (MIC) to drugs was determined in 98 strains of Mycobacteium tuberculosis (MTB) isolated in Tokyo from 2000 to 2003, to find which were resistant to any of the four main anti-MTB drugs, isoniazid (INH), rifampicin (RFP), streptomycin (SM), and ethambutol (EMB). 27strains of them were resistant only to SM, and 16 strains were resistant only to INH. 51 strains of them were resistant to not only INH but also other drugs. 38 strains were resistant to both INH and RFP. 19 strains were resistant to all four drugs, including 7 strains resistant to new quinolon anti-biotics also.<BR>Nucleotide or amino-acid mutations in drug resistant MTB genome were determined by DNA sequencing method. Mutation of codon 516, 526, or 531 of rpoB gene was detected in 98% of MTBs resistant to RFP. Deletion or insertion of katG gene or nucleotide mutation at regulatory region of ahpC gene was detected in MTBs highly resistant to INH. Amino acid mutation of katG gene, especially at codon 315, was detected in MTBs resistant to INH intermediate. Nucleotide mutations at regulatory region of inhA gene were detected in MTBs resistant to INH at low level. Amino acid mutation at codon 43 or 88 of rpsL gene was detected in MTBs highly resistant to SM, and nucleotide mutation at 512, 513, or 516 of rrs gene was detected in MTBs resistant to SM at low level. Amino acid mutation at codon 306 of embB gene was detected in 87% of MTBs resistant to EMB.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 79 (6), 388-396, 2005

    The Japanese Association for Infectious Diseases

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