Identification of Neisseria Gonorrhoeae by Co-agglutination Test

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  • Co-agglutination Testによるリン菌の同定
  • Co agglutination Test ニヨル リンキン ノ ドウテイ

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A slide co-agglutination test was perfomrd, using a serological identification reagent “Phadebact ® Gonococcus Test”(Pharmacia Diagnostic AB: Sweden) with 120 strains of Neisseria gonorrhoeae, 42 strains of N. meningitidis, 19 strains of other Neisseria species, 6 strains of Branhamella catarrhalis and 2 strains of Haemophilus influenzae.<BR>The Menck's direct colonies method, by which a few trypsin treated colonies of organisms grown on serum-free medium were mixed directly with the reagent, produced 10% of non-interpretable results among 80 strains of N. gonorrhoeae. All of the non-interpretable were predominated with T2 colony. In addition 11% of N. gonorrhoeae produced negative results by this method, and all the neagative strains were constituted of T4 colony only.<BR>On the other hand, a new procedure of boiling colonies method, described by the manufacturer, by which the organisms suspended in distilled water were heated at 100°C for 5 min prior to testing, produced positive results in all strains of N. gonorrhoeae examined. No positive results were obtained with 69 sti ains of non-gonococcal isolate by either method. However the number of non-interpretable was reduced and that of negative was increased by boiling colonies method.<BR>Using 120 strains of N. gonorrhoeae a comparison was made between co-agglutination (COA) test and conventional biological identification test by the Cystine Trypticase Agar (CTA) sugar degradation procedure. All strains of N. gonorrhoeae produced positive results by the COA test, while 2 strains 1.7% did not degrade glucose by the CTA procedure.<BR>The fresh clinical isolates as well as stock strains of N. gonorrhoeae produced positive results by the COA test, excepting for some few strains constituting of T4 colony only which produced somewhat weak postitive reaction.<BR>Using the colonies of oxidase positive and Gram-negative cocci primarily isolated on selective medium, or colonies transferred on non-selective media, a rapid identification by COA test alon was possible, except for a few non-gonococcal colonies with non-interpretable results which needed biological identification.<BR>Though there are some problem with respect to price and supply route for the reagent, the COA test is considered to be a very convenient, practical and accurate serological identification method for N. gonorrhoeae even for small bacteriological laboratories.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 54 (10), 529-537, 1980

    The Japanese Association for Infectious Diseases

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