Analysis of an Antigen in <I>Chlamydia trachomatis</I> Infected-cell Extract which Reacts with Sera of Patients with <I>C. trachomatis</I> Infection

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  • SATO Kozue
    Department of Medical Technology, School of Health Sciences, Kyorin University
  • KOMODA Teruko
    Department of Medical Technology, School of Health Sciences, Kyorin University
  • BANNAI Hisaichi
    Department of Medical Technology, School of Health Sciences, Kyorin University
  • AKITA Hironobu
    Department of Pediatrics, St-Marianna University of Medicine, Yokohama City Seibu Hospital
  • IWATA Satoshi
    Department of Pediatrics, National Tokyo Medical Center
  • SATO Yoshitake
    Department of Pediatrics, Fuji Heavy Industries LTD. Health Insurance Society General Ohta Hospital
  • SUNAKAWA Keisuke
    Department of Infectious Diseases, School of Medicine, Kitasato University

Bibliographic Information

Other Title
  • <I>Chlamydia trachomatis</I>感染細胞抽出液に見出された<I>C.trachomatis</I>感染患者血清と反応する抗原の解析
  • Chlamydia trachomatis感染細胞抽出液に見出されたC.trachomatis感染患者血清と反応する抗原の解析
  • Chlamydia trachomatis カンセン サイボウ チュウシュツエキ ニ ミイダサレタ C trachomatis カンセン カンジャ ケッセイ ト ハンノウ スル コウゲン ノ カイセキ
  • Analysis of an Antigen in Chlamydia trachomatis Infected-cell Extract which Reacts with Sera of Patients with C. trachomatis Infection

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Abstract

To find a new marker for sero-diagnosis of Chlamydia trachomatis infection, Western blot (WB) method was performed by using C. trachomatis infected HeLa229 cell extract (L2-ext) as antigen. Two series of sera of patients with pneumonia or cervicitis (4 sera each) were used for investigation and analysis of a band which fades earlier than others depending on weeks or antibody levels after therapeutic treatment. A 17KDa band was found which tended to fade gradually, but did not completely disappear within the period of investigation.<BR>The band was also detected in sera of patients with cervicitis diagnosed by detection of C. trachomatis organisms (IDEIA-PCE Chlamydia (IDEIA) or Clearview test were positive at first visit to the clinic). Ten anti-C. pneumoniae antibody positive sera and five anti-Mycoplasma pneumoniae antibody positive sera were also tested as controls. The result was that a 17KDa band was detected in 20 of 25 sera (80%) with IDEIA positive-, 18 of 33 patients (54.5%) with Clearview positive-, and 12 of 16 (75%) sera with both tests positive. No band was found in the control sera. The frequency of antibody against 17KDa antigen was almost completely identical with that obtained by microimmunofluorescence test and Peptide-Chlamydia-IgG test. These results show that a 17KDa band may be used as a marker for detection of C. trachomatis antibody by the WB method.<BR>The antigen could be precipitated with salting out from the L2-ext with 60% saturation of ammonium sulfate of Hofmeister's method and it was digested with proteinase K. From the result of the amino acid sequence analysis, it was found that 17KDa protein is the human nucleoside diphosphate kinase B.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 77 (10), 830-838, 2003

    The Japanese Association for Infectious Diseases

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