The New Screening Method of Beta Hemolytic Streptococcusby Dextrose Disk

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  • ブドウ糖デイスク法によるA群溶連菌の確認法
  • ブドウトウ デイスクホウ ニ ヨル Aグン ヨウレンキン ノ カクニンホウ

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In the diagnosis of streptococcal infection, it is especially important to isolate group A streptococcusin relation to the prognostic consideration; rheumatic fever, acute glomerular nephritis and heart disease. And, the study was subjected on the hemolysis inhibition test by Kobayashi as a screening method inthe WHO Expert Comittee 1967. This study aimed to enhance the practicability of Kobayashi methodby dextrose disk.<BR>The principle of Kobayashi method is based on the hemolysis inhibition by dextrose on the bloodagar but the minimum concentration of dextrose for this test is not quite clear. The results of this studyon the total of 550 strains of beta hemolytic streptococci including stocked and freshly isolated strainsin the field works indicate that 1.2mg per disk is most suitable for the screening of group A streptococcus.<BR>In this test the strains to be tested were inoculated by stub method at 2 different places which are 2mm and 15mm distance from the disk. At the place of 2mm distance from the disk, the beta hemolysiswas inhibited but not at the place of 15mm distance.<BR>This is a simpler and more reliable method for the hemolysis inhibition test of beta hemolyticstreptococcus. Moreover, in this test streptococcus could be divided into such 3 groups as: Group I: the hemolysis is strongly inhibited by dextrose (Group A, Lancefield) Group II: the pigment sedimentation due to dextrose is observed (Group B, Lancefield) Group III: no change in himolysis by dextrose (Other than Group A and B, Lancefield).

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 48 (11), 441-448, 1974

    The Japanese Association for Infectious Diseases

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