Identification of <I>Vibrio vulnificus</I> from Patients with Otitis Media and Septicemia by PCR Method

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  • SATO Susumu
    Department of Medical Technology, School of Health Sciences, Hirosaki University
  • MIURA Tomisato
    Department of Medical Technology, School of Health Sciences, Hirosaki University
  • SAITOH Masaaki
    Adult Diseases Examination Center, Hirosaki Medical Association
  • TSUKIASHI Seishin
    Adult Diseases Examination Center, Hirosaki Medical Association
  • HONGO Toshiharu
    Department of Laboratory Medicine, Kurashiki Central Hospital
  • TOBA Takahiro
    Department of Biochemistry and Biotechnology, Faculty of Agriculture, Hirosaki University

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Other Title
  • 中耳炎および敗血症例から分離された<I>Vibrio vulnificus</I>のPCR法による同定
  • 中耳炎および敗血症例から分離されたVibrio vulnificusのPCR法による同定
  • チュウジエン オヨビ ハイケツショウレイ カラ ブンリ サレタ Vibrio vulnificus ノ PCRホウ ニ ヨル ドウテイ
  • Identification of Vibrio vulnificus from Patients with Otitis Media and Septicemia by PCR Method

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Abstract

Sucrose-negative colonies were isolated on TCBS agar plate from otorrhea specimens from otitismedia patient in the Adult Diseases Examination Center, Hirosaki Medical Association. The isolatewas identified as Vibrio vulnificus by nested PCR method which amplify V. vulnificus-specific sequencesdirected against 23S rRNA genes. The PCR method was applied to identify 6 strains originallyisolated from septicemia patients in Kurashiki Central Hospital and formerly identified as V.vulnificus by phenotypic characteristics. When examined by the API20E system, the above PCRconfirmed-V. vulnificus isolates were correctly identified as V. vulnificus with % ID 99.8, though thisgave 3 different profiles. Cytotoxin-hemolysin gene was detected in otorrhea strain as well as septicemiastrains by PCR method. The above results suggest that PCR method targeted cytotoxinhemolysingene is suitable for rapid and accurate identification of the isolate, because the result is obtainedin less than 4h.<BR>To our knowledge this is the first report on the V. vulnificus infection in Aomori Prefecture andthe isolation from otorrhea.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 75 (4), 307-313, 2001

    The Japanese Association for Infectious Diseases

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