Comparison of Detection Sensitivity in Rapid-diagnosis Influenza Virus Kits

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  • 各種インフルエンザ迅速診断キットの評価 ―検出感度の比較検討―
  • カクシュ インフルエンザ ジンソク シンダン キット ノ ヒョウカ ケンシュツ カンド ノ ヒカク ケントウ

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Description

Rapid-diagnosis kits able to detect influenza A and B virus by immunochromatography developed by different manufacturers, while useful in early diagnosis, may vary widely in detection sensitivity. We compared sensitivity results for eight virus-detection kits in current use-Quick Chaser FluA, B Ⓡ(Mi zuho Medy), Espline ⓇInfluenza A & B-N (Fujirebio), Capilia ⓇFlu A＋B (Nippon Beckton Dickinson & Alfesa Pharma), Poctem ⓇInfluenza A/B (Otsuka Pharma & Sysmex), BD Flu Examan Ⓡ(Nippon Beckton Dickinson),Quick Ex-Flu Ⓡ“Seiken”(Denka Seiken), Quick Vue ⓇRapid SP Influ (DP Pharma Biomedical), and Rapid Testa ⓇFLU stick (Daiichi Pure Chemicals)-against influenza virus stocks, contained five vaccination strains (one A/H1N1, two A/H3N2, and two B) and six clinical strains (two A/H1N1, two A/H3N2, and two B). Mini mum detection concentrations giving immunologically positive signals in serial dilution and RNA copies in positive dilution in real-time reverse transcriptase-polymerase chain reaction (RT-PCR) were assayed for all kits and virus stock combinations. RNA log10 copy numbers/mL in dilutions within detection limits yielded 5.68-7.02, 6.37-7.17, and 6.5-8.13 for A/H1N1, A/H3N2, and B. Statistically significant differences in sensitivity were observed between some kit combinations. Detection sensitivity tended to be relatively higher for influenza A than B virus. This is assumed due to different principles in kit methods, such as monoclonal antibodies, specimen-extraction conditions, and other unknown factors.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 83 (5), 525-533, 2009

    The Japanese Association for Infectious Diseases

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