Biotyping of <I>Campylobacter jejuni</I> Isolated from Diarrheal Patients

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  • FUKAMI Tosie
    Department of Clinical Laboratory, Tokyo Metropolitan Komagome Hospital

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  • 下痢患者より検出された<I>Campylobacter jejuni</I>の生物型分類について
  • 下痢患者より検出されたCampylobacter jejuniの生物型分類について
  • ゲリ カンジャ ヨリ ケンシュツサレタ Campylobacter jejun
  • Biotyping of Campylobacter jejuni Isolated from Diarrheal Patients

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Abstract

Several biotyping schemes of Campylobacter jejuni have been proposed. A scheme of Skirrow andBenjamin (J. Clin. Pathol., 33, 1122, 1980) is based on hippurate hydrolysis and rapid H2S test. Hebert (J. Clin. Microbiol., 15 1065, 1982) has proposed a scheme based on hippurate hydrolysis, DNA hydrolysis and growth on charcoal yeast extract agar. Recently, Lior (J. Clin. Microbiol., 20, 636, 1984) has proposed a new scheme using improved media and methods for the detection of hippurate hydrolysis, rapid H2S production and DNA hydrolysis.<BR>Following Lior's scheme, attempts were made to do biotyping 307 strains of Campylobacter jejuniisolated from diarrheal patients in our hospital. As reported by Lior, four biotypes were identified, 178 strains (58.0%) belonged to biotype I, 122 strains (39.7%) to biotype II, 3 strains (1.0%) to biotype III and 4 strains (1.3%) to biotype IV.<BR>It is concluded that the Lior's biotyping scheme is easy to carry out and is useful for epidemiological study of Campylobacter jejuni infections.

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  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 60 (4), 299-303, 1986

    The Japanese Association for Infectious Diseases

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