Mutation of Penicillin-binding Protein Genes and Antimicrobial Susceptibility of <I>Haemophilus influenzae</I> Isolated from Spinal Fluid or Blood in Children

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  • 小児の血液・髄液から分離された<I>Haemophilus influenzae</I>のpenicillin結合蛋白遺伝子変異と薬剤感受性
  • 小児の血液・髄液から分離されたHaemophilus influenzaeのpenicillin結合蛋白遺伝子変異と薬剤感受性
  • ショウニ ノ ケツエキ ズイエキ カラ ブンリ サレタ Haemophilus influenzae ノ penicillin ケツゴウ タンパク イデンシ ヘンイ ト ヤクザイ カンジュセイ
  • Mutation of Penicillin-binding Protein Genes and Antimicrobial Susceptibility of Haemophilus influenzae Isolated from Spinal Fluid or Blood in Children

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Abstract

Between September 1999 and August 2001, we studied serotypes to capsular antigen, betalactamase production, mutation of penicillin binding protein (PBP) genes by PCR method, and antimicrobial susceptibilities of 13 strains of Haemophilus influenzae isolated from spinal fluid or blood in children. Diseases of patients were meningitis in 11, pneumonia in 1, and laryngitis in 1. The age range of the patients was from 26 days to 5 years. The serotypes of all strains were b. Four of the 13 strains were beta-lactamase-positive. The mutation of genes of pbp3 was revealed from 4 isolates and 2 of the strains were beta-lactamase-positive. MICs of ampicilin to beta-lactamase-negative strains ranged from 0.125 to 1μg/eml and those to beta-lactamase-positive were more than 32μg/ml. MICs of 2 strains of beta-lactamase-negative and mutation-positive were 0.5 and 1μg/ml. The excellent active antimicrobials in our study was cefotaxime (MIC90 0.06μg/ml), meropenem (MIC90 0.125μg/ml), ceftazidime (MIC90 0.25μg/ml), and cefepime (MIC90 0.25μg/ml).

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 76 (4), 280-284, 2002

    The Japanese Association for Infectious Diseases

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