Gene Mutations Associated with Macrolide-resistance and p1 Gene Typing of Mycoplasma pneumoniae Isolated in Yamagata, Japan, between 2004 and 2013

SUZUKI Yu, SETO Junji, ITAGAKI Tutomu, AOKI Toshiya, ABIKO Chieko, MATSUZAKI Yoko

doi:10.11150/kansenshogakuzasshi.89.16

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山形県で2004 年から2013 年の10 年間に分離したMycoplasma pneumoniae のマクロライド耐性遺伝子変異およびp1 遺伝子型解析
山形県で2004年から2013年の10年間に分離したMycoplasma pneumoniaeのマクロライド耐性遺伝子変異およびp1遺伝子型解析
ヤマガタケンデ 2004ネンカラ 2013ネンノ 10ネンカンニブンリシタ Mycoplasma pneumoniae ノマクロライドタイセイイデンシヘンイオヨビ p1 イデンシガタカイセキ
Gene mutations ssociated with macrolide-resistance and p1 gene typing of Mycoplasma pneumoniae isolated in yamagata, japan, between 2004 and 2013.
Gene mutations associated with macrolide-resistance p1 gene typing of Mycoplasma pneumoniae isolated in Yamagata, Japan, between 2004 and 2013

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Abstract

To clarify the epidemiologic features of Mycoplasma pneumoniae, we examined 358 M. pneumoniae strains isolated between 2004 and 2013 in Yamagata, Japan. Analysis of macrolide-resistance-associated 23S ribosomal RNA (rRNA) domain V mutations revealed 6 kinds of mutants (81 A2063G, 43 A2063T, 1 A2063C, 1 A2064C, 4 C2617G and 1 C2617 mutation). There were only two mutants before 2009, but mutants A2063T and A2063G increased in 2009 and from 2010, respectively. The annual ratio of mutants varied from 20.4％to 76.4％between 2009 and 2013. Typing of the p1 gene revealed 4 types ; 278 type 1, and 3 kinds of type 2 variant strains (10 type 2a, 5 type 2b and 65 type 2c). Type 1 strains accounted for between 85.2％and 100％of isolates from 2004 to 2011, whereas type 2 variant strains increased by 26.5％and 66.1％in 2012 and 2013, respectively. These results indicate that type 1 strains may have been replaced by type 2 variant strains in 2013. Furthermore,the ratio of type 1 strains with a 23S rRNA mutation was 65.1％in 2012 and 95.2％in 2013, but none of the type 2 variant strains had this mutation. In conclusion, type 1 strains with macrolide-resistant mutations appeared in 2006 and increased from 2009. In contrast, type 2 variant strains, which increased in 2012 and became predominant in 2013, showed no mutations.

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Kansenshogaku Zasshi

Kansenshogaku Zasshi 89 (1), 16-22, 2015

The Japanese Association for Infectious Diseases

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Gene Mutations Associated with Macrolide-resistance and <i>p1 </i>Gene Typing of <i>Mycoplasma pneumoniae </i>Isolated in Yamagata, Japan, between 2004 and 2013