Antimicrobial Susceptibility and Resistance Mutations in <i>Campylobacter jejuni </i>and <i>C. coli </i>Isolates from Human and Meat Sources

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  • OISHI Akira
    Department of Health Science, Fukuoka Institute of Health and Environmental Sciences
  • MURAKAMI Koichi
    Department of Health Science, Fukuoka Institute of Health and Environmental Sciences
  • ETOH Yoshiki
    Department of Health Science, Fukuoka Institute of Health and Environmental Sciences
  • SERA Nobuyuki
    Department of Health Science, Fukuoka Institute of Health and Environmental Sciences
  • HORIKAWA Kazumi
    Department of Health Science, Fukuoka Institute of Health and Environmental Sciences

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Other Title
  • 食肉およびヒトの便から分離した<i>Campylobacter jejuni</i>/<i>coli </i>の 薬剤感受性試験並びに耐性遺伝子変異の検討
  • 食肉およびヒトの便から分離したCampylobacter jejuni/coliの薬剤感受性試験並びに耐性遺伝子変異の検討
  • ショクニク オヨビ ヒト ノ ベン カラ ブンリ シタ Campylobacter jejuni/coli ノ ヤクザイ カンジュセイ シケン ナラビニ タイセイ イデンシ ヘンイ ノ ケントウ

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Abstract

Recently, there has been a marked increase in the number of reports of fluoroquinolone-resistant Campylobacter jejuni and Campylobacter coli. The aim of this study was to evaluate the prevalence of antimicrobial resistance and its genetic determinants in Campylobacter species isolated from meat and human subjects in Fukuoka Prefecture, Japan. Between 2011 and 2013, 55 and 64 isolates were collected from meat (chicken meat and beef liver) and humans, respectively, in this prefecture. Antimicrobial susceptibility tests were conducted using the agar dilution method in accordance with the Clinical and Laboratory Standards Institute guidelines, using the following 11 antimicrobial agents： cephalexin, cefoxitin, nalidixic acid,ciprofloxacin, levofloxacin, tetracycline, minocycline, ampicillin, streptomycin, kanamycin and erythromycin. The susceptibility rates of the isolates to three quinolones (nalidixic acid, ciprofloxacin, levofloxacin) were 43.7%, 41.2%, 40.3%, respectively. All the isolates were multidrug resistant. Whereas 46.9%-51.6% of the human isolates were resistant to one or more of the quinolones, only 32.7%-34.5% of the meat isolates were resistant to one or more of the drugs. DNA sequencing showed that of the 50 quinolone resistant isolates 44 had position 86 isoleucine (Ile) substituted for threonine (Thr) in the GyrA protein (Thr86Ile). This amino acid substitution resulted from ACA to ATA and ACT to ATT mutations of codon 86 in C. jejuni and C. coli, respectively. Furthermore, two of the four C. jejuni isolates lacking the Thr86Ile mutation had combined Ser22Gly-Asn203Ser substitutions, while the remaining two isolates had combined Ser22Gly-Asn203Ser-Ala 206Val substitutions. These four isolates also had cmeABC sequences that differed from the quinolone sensitive C. jejuni ATCC33560T strain. In conclusion, C. jejuni and C. coli have relatively high quinolone resistance,and are resistant to other antibiotics. The new combination of amino acid substitutions in the GyrA protein could pose a potential threat to public health in Japan.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 89 (2), 244-253, 2015

    The Japanese Association for Infectious Diseases

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