Effect of Human Serum Albumin on Endotoxin Scattering Photometry
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- TAKAHASHI Gaku
- Department of Critical Care Medicine, Iwate Medical University
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- SHIBATA Shigehiro
- Department of Critical Care Medicine, Iwate Medical University
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- KAN Shigenori
- Department of Critical Care Medicine, Iwate Medical University
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- INADA Katsuya
- Department of Critical Care Medicine, Iwate Medical University
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- ENDO Shigeatsu
- Department of Critical Care Medicine, Iwate Medical University
Bibliographic Information
- Other Title
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- エンドトキシン散乱測光法におけるヒト血清アルブミンの影響
- エンドトキシン サンラン ソッコウホウ ニ オケル ヒト ケッセイ アルブミン ノ エイキョウ
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Abstract
Purpose : Laser scattering photometry (ESP) is a newly developed plasma endotoxin assay method using horseshoe crab amebocyte lysate (AL) that recognizes small particles produced by polymerization of coagulin under the stirring conditions at 1000 rpm. We elucidated the effect of human serum album (HSA) in the ESP method. Methods : AL was dissolved with 630μL of the specimen and a 200-μL aliquot was used for ESP ; this conventional protocol was regarded as the ESP 630 method. The ESP 210 method was also used, i. e. AL was dissolved with 210μL of the specimen and a 200-μL aliquot was used for ESP. Results : Water induced the agglutination, and HSA prolonged the agglutination time depending on its concentration especially in the ESP630 method. The water-induced agglutination was not inhibited by the addition of anti-factor C monoclonal antibody, and amidinophenyl benzoate hydrochloride, used as a clotting enzyme inhibitor, intensively inhibited the water-induced agglutination. Therefore, the water-induced agglutination was suggested to be a false-positive reaction to non-specific activation of the clotting enzyme. The HSA-induced prolongation of the reaction in the national health insurance-covered turbidimetric kinetic assay was not observed. Conclusion : HSA or plasma protein seemed to affect the result, especially in the ESP630 method, and a non-specific reaction was found to occur in the ESP methods.
Journal
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- Kansenshogaku Zasshi
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Kansenshogaku Zasshi 89 (4), 452-457, 2015
The Japanese Association for Infectious Diseases
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Details 詳細情報について
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- CRID
- 1390282680028353536
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- NII Article ID
- 130005868581
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- NII Book ID
- AN00047715
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- ISSN
- 1884569X
- 03875911
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- NDL BIB ID
- 026637095
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed