The principle of LAMP method-A simple and rapid gene amplification method-

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Other Title
  • ＬＡＭＰ法の原理‐遺伝子の簡易・迅速な増幅法‐
  • LAMP法の原理--遺伝子の簡易・迅速な増幅法
  • LAMPホウ ノ ゲンリ イデンシ ノ カンイ ジンソク ナ ゾウフクホウ
  • <b>—A simple and rapid gene amplification method—</b>
  • <b>—遺伝子の簡易・迅速な増幅法—</b>

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Abstract

So far nucleic acid test (NAT) has been employed in various fields, including infectious disease diagnoses. However, due to its complicated procedures and relatively high cost, it has not been widely utilized in many actual diagnostic applications. We have therefore developed a simple and rapid gene amplification technology, Loop-mediated Isothermal Amplification (LAMP) method, which has shown prominent results of surpassing the performance of the conventional gene amplification methods. LAMP method acquires three main features : (1) all reaction can be carried out under isothermal conditions ; (2) the amplification efficiency is extremely high and tremendous amount of amplification products can be obtained ; and (3) the reaction is highly specific. Furthermore, developed from the standard LAMP method, a rapid LAMP method, by adding in the loop primers, can reduce the amplification time from the previous 1 hour to less than 30 minutes. Enormous amount of white precipitate of magnesium pyrophosphate is produced as a by-product of the amplification, therefore, direct visual detection is possible without using any reaction indicators and detection equipments. We believe LAMP technology, with the integration of these features, can rightly apply to clinical genetic testing, food and environmental analysis, as well as NAT in different fields.

Journal

  • Uirusu

    Uirusu 54 (1), 107-112, 2004

    The Japanese Society for Virology

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