Long Term Monitoring of Bacterial Community Succession in Septic Tank by Denaturing Gradient Gel Electrophoresis of 16S rRNA Gene Fragments
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- KOIZUMI Yoshikazu
- Tamagawa Gakuen Branch, Environmental Engineering Center Co., Ltd. Applied Life Science Research Center, Tamagawa University Research Institute
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- MIZUTANI Atsushi
- Faculty of Agriculture, Tamagawa University
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- MITSUI Ayako
- Tamagawa Gakuen Branch, Environmental Engineering Center Co., Ltd.
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- YOSHIMURA Yoshitaka
- Faculty of Agriculture, Tamagawa University
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- FUKUI Manabu
- The Institute of Low Temperature Science, Hokkaido University
Bibliographic Information
- Other Title
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- 浄化槽の処理プロセスにおける真正細菌群集の変性剤濃度勾配ゲル電気泳動法による長期間モニタリング
- ジョウカソウ ノ ショリ プロセス ニ オケル シンセイ サイキン グンシュウ ノ ヘンセイザイ ノウド コウバイ ゲル デンキ エイドウホウ ニ ヨル チョウキカン モニタリング
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Abstract
The long-term succession of bacterial communities in a medium-scale septic tank was monitored by the denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA fragments. The calculation of diversity indices from DGGE profiles indicated that the bacterial diversity in the aeration tank was significantly higher than those in the other tanks studied. Ten of the 23 sequenced DGGE bands were affiliated with beta-proteobacteria and some of these bacteria were prominent. Clustering analysis of the DGGE banding pattern revealed that spikes in feed strength caused significant changes in community structure in the raw water and aeration tanks. Dendrogram construction using the DGGE profiles of the aeration tank showed that the bacterial community structure changed by direct inputs of organic waste matter returned to the original stable community structure over time. The specification of bacteria that mainly affect the succession of the indigenous bacterial community structure was facilitated by the dendrogram construction from DGGE profiles.
Journal
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- Journal of Japan Society on Water Environment
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Journal of Japan Society on Water Environment 31 (2), 109-116, 2008
Japan Society on Water Environment
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Keywords
Details 詳細情報について
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- CRID
- 1390282680057757056
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- NII Article ID
- 10021158292
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- NII Book ID
- AN10372439
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- COI
- 1:CAS:528:DC%2BD1cXjsFahtLk%3D
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- ISSN
- 18813690
- 09168958
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- NDL BIB ID
- 9376645
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed