Determination of Drug Concentrations in Biological Fluids by Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry(GC/MS)

DOI Web Site Open Access
  • NODA Hiroshi
    Department of Hospital Pharmacy, School of Medicine, University of Occupational and Environmental Health
  • NODA Atsuko
    Faculty of Pharmaceutical Sciences, Kyushu University
  • MATSUYAMA Kenji
    Faculty of Pharmaceutical Sciences, Kyushu University
  • YEN Shu-Hwai
    Faculty of Pharmaceutical Sciences, Kyushu University
  • IGUCHI Sadao
    Faculty of Pharmaceutical Sciences, Kyushu University

Bibliographic Information

Other Title
  • ガスクロマトグラフィー（GC）およびガスクロ質量分析法（GC/MS）による体液中医薬品濃度の定量

Search this article

Description

For clinical monitoring drug therapy, saliva is an useful body fluid. However prior to employing saliva for this purpose it is necessary to elucidate the behaviors of drugs in saliva in order to establish adequate methods in detecting drug concentrations. This time the fate of hydralazine (HP) in human saliva was examined. By means of GC and GC/MS, transformation of HP to tetrazolo[5, 1-a] phthalazine (Tetra-P) as a main product in human saliva or in NaNO2-administered rabbit urine was observed under simulated gastric conditions. In acid free saliva, 3-methyl-s-triazolo[3, 4-a] phthalazine (MTP) and s-triazolo[3, 4-a] phthalazine (Tri-P), both derived from acetylated HP, were the main products after incubation at 37℃. The results show that the amounts of the products-MTP, Tri-P and Tetra-P-depend considerably on pH and incubation time. It also should be noted that hydrazine (HZ), a carcinogen as well as a mutagen, was detected as one of the metabolites of HP in rabbit urine. Reaction course for the formation of Tetra-P in human saliva under simulated gastric condition was also discussed.

Journal

  • Journal of UOEH

    Journal of UOEH 1 (3), 339-349, 1979

    University of Occupational and Environmental Health, Japan

Keywords

Details 詳細情報について

Report a problem

Back to top