The Load Effect of Electric Current on the Lowering of Intracellular pH in Plant with the Progress of Cooling

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  • NINAGAWA Takako
    Graduate School of Mechanical Engineering, Kanagawa Institute of Technology
  • NARUMI Akira
    Department of Mechanical Engineering, Kanagawa Institute of Technology
  • KAWAMURA Yukio
    Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University
  • KONISHI Tadashi
    Department of Mechanical Engineering, Oita National College of Technology

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Other Title
  • 冷却に伴う植物の細胞内pH低下への電流負荷効果
  • レイキャク ニ トモナウ ショクブツ ノ サイボウ ナイ pH テイカ エ ノ デンリュウ フカ コウカ

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Cryopreservation technique is very essential to the various fields, such as medical and food engineering. Considering the worldwide food shortage due to the explosive increase of world population and frequently occurring extraordinary climate in near future, the development of cryopreservation technique is desired. Under the above background, we have continued to investigate the load effect of electric current on plant cell in the cooling and freezing processes in order to discuss the possibility of new cryopreservation technique. We had found that the load of electric current influenced on the ice crystal behavior and temperature at the occurrence of intracellular freezing and reported about the results previously. In this research, we investigated how the load of electric current influenced on intracellular pH in the cooling process, focusing on intracellular pH which is one of indicators of cell activity. Helianthus tuberosus L. and Allium cepa L. were used as plants for testing. Intracellular pH was measured using fluorescence intensity ratiometry. As a result, it is clear that the load of electric current causes the reduction in the lowering of intracellular pH in plant with the progress of cooling.

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