線虫におけるAFGP の凍害防御効果

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  • Cryoprotective Effect of AFGP on the Nematodes
  • センチュウ ニ オケル AFGP ノ トウガイ ボウギョ コウカ

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For the investigation of cryoprotective effect of antifreeze glycoprotein (AFGP) on the nematode Caenorhabditis elegans and the Antarctic nematode Panagrolaimus davidi, we observed the freezing behavior and examined survival rate after freeze-thawing of the nematodes. When the Antarctic nematode P. davidi was cooled slowly to -75°C in the presence of 0.5% AFGP dissolved in M9 buffer, percentage of the unfrozen nematodes increased and most of them survived after thawing. In the case of nematode C. elegans that was cooled slowly, the nematodes in AFGP dissolved in M9 buffer could not survive though most of the nematodes in 0.5% AFGP dissolved in 5% DMSO could survive after freeze-thawing. The cryoprotective effect of AFGP was observed even when P. davidi was quenched by dipping it into LN2. When the nematodes were dipped in 0.5% AFGP dissolved in 5% DMSO and quenched with a cryo-plate, 79% of them could survive after thawing. Thus, it is clear that AFGP has cryoprotective effect on the nematodes.

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