書誌事項
- タイトル別名
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- Cryoprotective Effect of Glycine Betaine on the Nematodes
- センチュウ ニ オケル グリシンベタイン ノ トウガイ ボウギョ コウカ
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For the investigation of cryoprotective effect of glycine betaine (GB) on the nematode Caenorhabditis elegans and the Antarctic nematode Panagrolaimus davidi, we observed the freezing behavior and examined survival rate after freeze-thawing of the nematodes. When the nematode P. davidi was cooled slowly to -75℃ in the presence of GB (0.2 M - 1.0 M), most nematodes remained unfrozen and survived after thawing. However, when the nematodes were quenched with liquid nitrogen from -75℃, damaged nematodes were observed after thawing and survival rates were decreased. In the case of nematode C. elegans that was cooled slowly, percentage of the unfrozen nematodes and the survival rate after thawing increased as the GB concentration increased to 1.0 M. While the Antarctic nematode P. davidi was quenched by dipping into LN_2 from ambient temperature, the cryoprotective effect of GB was observed. When they dipped in 1.0 M GB were quenched with a cryo-plate, 73% of nematodes could survive after thawing. Thus, it was clear that GB has a cryoprotective effect on the nematodes.
収録刊行物
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- 低温生物工学会誌
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低温生物工学会誌 61 (2), 139-143, 2015
低温生物工学会
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詳細情報 詳細情報について
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- CRID
- 1390282680063705216
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- NII論文ID
- 110010001012
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- NII書誌ID
- AN10448734
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- ISSN
- 24241555
- 13407902
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- NDL書誌ID
- 026831695
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可