3. Freeze-drying Spermatozoa in Mice(Papers presented at the Seminar, "The latest developments in biopreservation technigues using low temperature and low moisture conditions", July 23, 2007, Tokyo)

DOI IR Web Site 36 References
  • SUZUKI Hiroshi
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine:Department of Developmental and Medical Technology, Graduate School of Medicine, The University of Tokyo
  • KAWASE Yosuke
    Chugai Research Institute for Medical Science Inc.

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  • 3. 凍結乾燥によるマウス精子の保存(セミナー「低温と乾燥による生物保存技術への挑戦～マミーエンジニアリング」)
  • 凍結乾燥によるマウス精子の保存
  • トウケツ カンソウ ニ ヨル マウス セイシ ノ ホゾン

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Abstract

Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of mouse spermatozoa is simpler, less time-consuming, and less costly than of embryos for preservation of the genetic materials, maintenance of cryopreserved spermatozoa still has a high running cost because of the need for a constant supply of liquid nitrogen. In 1998, it has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, attempts of freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried spermatozoa were reported in the cow and rabbit prior to 1960. Unfortunately, these preliminary results have not been confirmed. Spermatozoa become defective in motility after freeze-drying and are unable to fertilize eggs both in vivo and in vitro. Since freeze-drying of mammalian spermatozoa was demonstrated without loss of genetic or reproductive potential, recent investigations of freeze-dried sperm have focused on the factors affecting freeze-drying spermatozoa in an effort to store the male gamete at ambient temperature. It is particularly essential to assure long-term preservation for several decades or centuries. Recent findings indicate that the pressure levels at primary drying of freeze-dried spermatozoa, in addition to the components of the suspending solution, appears to be an important factor for long-term preservation of freeze-dried spermatozoa in mice.

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