Analysis of Urinary Metabolites of Rats Exposed to Ethylene Oxide

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  • KOGA Minoru
    Department of Environmental Health EngineeringInstitute of Industrial Ecological Sciences, University of Occupational and Environmental Health
  • HORI Hajime
    Department of Environmental Health EngineeringInstitute of Industrial Ecological Sciences, University of Occupational and Environmental Health
  • TANAKA Isamu
    Department of Environmental Health EngineeringInstitute of Industrial Ecological Sciences, University of Occupational and Environmental Health
  • AKIYAMA Takashi
    Department of Environmental Health EngineeringInstitute of Industrial Ecological Sciences, University of Occupational and Environmental Health
  • INOUE Naohide
    Department of Environmental Toxicology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health

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Other Title
  • 酸化エチレン暴露ラットにおける尿中代謝物の分析

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Description

Urinary metabolites of rats exposed to ethylene oxide (EO) were analyzed by gas chromatography-mass spectrometry (GC/MS). Male Wister rats (220－240 g) were exposed to EO for 6 hours. The urine was collected during 18 hours after the exposure and extracted with ethyl acetate. For the water-soluble metabolites, ethanol was added to the urine, centrifuged and the supernatant fraction was evaporated to dryness. The residue of the extract was methylated with diazomethane and trimethylsilylated with bis (trimethylsilyl) trifluoroacetamide. Ethylene glycol, 2-hydroxymercapturic acid, 2-methylthioethanol and 2-mercaptoethanol were identified as the metabolites of EO. These results suggest that the inhaled EO was hydrolyzed to ethylene glycol, and conjugated with glutathion to form the mercapturic acid and methylthio metabolite.

Journal

  • Journal of UOEH

    Journal of UOEH 9 (2), 167-170, 1987

    University of Occupational and Environmental Health, Japan

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