Physicochemical Traits of Cryptobiosis in Larvae of Polypedilum vanderplanki : Importance of Vitrification of Trehalose(Papers presented at the 51^<st> Annual Meeting)

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  • WATANABE Masahiko
    Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences
  • FURUKI Takao
    Center for Biological Resources and Informatics, Tokyo University of Technology
  • SAKURAI Minoru
    Center for Biological Resources and Informatics, Tokyo University of Technology
  • AKAO Kenichi
    Spectroscopic Instruments Division, JASCO Corporation
  • KIKAWADA Takahiro
    Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences
  • NAKAHARA Yuichi
    Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences
  • OKUDA Takashi
    Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences

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Other Title
  • ネムリユスリカの幼虫におけるクリプトビオシスの物理化学的性質 : トレハロースのガラス化の重要性(平成17年度 第51回低温低物工学会研究報告)
  • ネムリユスリカの幼虫におけるクリプトビオシスの物理化学的性質:トレハロースのガラス化の重要性
  • ネムリユスリカ ノ ヨウチュウ ニ オケル クリプトビオシス ノ ブツリカガクテキ セイシツ トレハロース ノ ガラスカ ノ ジュウヨウセイ

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Larvae of Polypedilum vandezplanki can enter the state of cryptobiosis in which organisms are almost completely dehydrated and the metabolic activity is undetectable. One of the most characteristic phenomena is remarkable accumulation of trehalose in the cryptobiotic larva. This study aimed to clarify an importance of vitrified trehalose in cryptobiosis. Slowly-dehydrated larva (Slow sample) accumulated much trehalose, successfully entered the state of cryptobiosis, and showed high temperature tolerance, whereas quickly-dehydrated one (Quick sample) with a small amount of trehalose failed to do. Differential scanning calorimetric (DSC) analysis demonstrated that glass transition took place only in Slow sample, suggesting that a large amount of trehalose in the larval body is actually vitrified. When Slow samples were exposed to high relative humidity such as 93 or 98% for 5 days, the glassy state was lost and the recovery rate was lowered, although the trehalose content did not change so much. On the other hand, those exposed to 38% or 60% r.h. maintained the glassy state and the ability for recovery. However, their glass-transition temperatures declined, and consequently they became sensitive to high temperature. The results in the present study suggest vitrification of trehalose in the larval body is essential for induction and maintenance of cryptobiosis in P. vanderplanki.

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