鼠癩菌の低酸素分圧下における培養

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  • Cultivation of <i>Mycobacterium lepraemurium</i> under Low Oxygen Tensions
  • ネズミライキン ノ テイ サンソ ブンアツカ ニ オケル バイヨウ

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Cytochrome bl and cytochrome a2 were detected in the in vivo grown or in vitro grown Mycobaterium lepraemurium, however, cytochrome c and cytochrome a were not found. Cytochrome a2 is a complex of D and C type cytochrome which is mainly seen in Pseudomonas aeruginosa grown in anaerobic condition and is not seen in the bacteria grown in aerobic condition. Mycobacterium lepraemurium was grown in aerobic condition on 1% Ogawa yolk medium, however, growing place of the organisms might be fairly anaerobic condition because the cytochrome a2 was found in this organisms. When a few bacteria was inoculated on the 1% Ogawa yolk medium, the organism might be unable to make an optimal anaerobic condition on this medium. Then trial of the cultivation of Mycobacterium lepraemurium under low oxygen tension was made by the author.<br>Media were put in glass desiccator and were exchanged with gas mixture through sponge gum cap. Mixture gas was added in desiccator once a weeks. As shown in Table 1, the mixture gas composed of 5% CO2, 1% O2 and 96% N2 gave the best result for cultivating with a few organism, but no colonie formation was obtained in case of the inoculation with 105 bacilli. In the primary isolation of Mycobacterium lepraemurium 100% success was not obtained, especially primary isolation from infectious tissue contained relatively little numbers of bacilli was very difficult. As seen in Table 2, the low oxygen condition was better than normal air condition. Primary isolation from ten times diluted inoculum was failed in normal air condition, but succeeded on some tubes in 1% condition. Primary isolation of Mycobacterium lepraemurium from tissue culture of A 31 cells which contained less bacilli than the murine leproma, is also possible in 1% O2 condition.<br>Acknowledgment.<br>In this work, the author was indebted to Miss Saito for making the media. This work was supported in part by the grants from World Health Organization and the USJAPAN Cooperative Medical Science Program.

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