- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Automatic Translation feature is available on CiNii Labs
- Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
Double Transgenic Mice Crossed GFP-LC3 Transgenic Mice With .ALPHA.MyHC-mCherry-LC3 Transgenic Mice Are a New and Useful Tool to Examine the Role of Autophagy in the Heart
-
- Terada Mai
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Nobori Kiyoshi
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Munehisa Yoshiko
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Kakizaki Manabu
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Ohba Takayoshi
- Department of Physiology, Akita University Graduate School of Medicine
-
- Takahashi Yoichiro
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Koyama Takashi
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Terata Yutaka
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Ishida Masaru
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Iino Kenji
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Kosaka Toshimitsu
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Watanabe Hiroyuki
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Hasegawa Hitoshi
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
-
- Ito Hiroshi
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
Search this article
Description
Background: The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice. Methods and Results: We generated transgenic mice expressing mCherry-LC3 under αMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation. Conclusions: This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74: 203 - 206)<br>
Journal
-
- Circulation Journal
-
Circulation Journal 74 (1), 203-206, 2010
The Japanese Circulation Society
