Heparin Induces the Mobilization of Heart-Derived Multipotent Mesoangioblasts During Cardiac Surgery With Cardiopulmonary Bypass or Cardiac Catheterization
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- Hata Yoshihiro
- Department of Medicine II, Kansai Medical University
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- Iwasaki Masayoshi
- Department of Medicine II, Kansai Medical University
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- Fujitaka Keisuke
- Department of Medicine II, Kansai Medical University
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- Park Haengnam
- Department of Medicine II, Kansai Medical University
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- Sato Daisuke
- Department of Medicine II, Kansai Medical University
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- Enoki Chiharu
- Department of Cardiovascular Surgery, Kansai Medical University
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- Minato Naoki
- Department of Cardiovascular Surgery, Kansai Medical University
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- Horitani Keita
- Department of Medicine II, Kansai Medical University
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- Nakano Miyuki
- Department of Medicine II, Kansai Medical University
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- Kishimoto Hiroshi
- Department of Medicine II, Kansai Medical University
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- Wada Kensaku
- Department of Medicine II, Kansai Medical University
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- Koyanagi Masamichi
- Kyushu University Beppu Hospital
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- Adachi Yasushi
- Division of Surgical Pathology, Toyooka Hospital
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- Yamamoto Yoshihiro
- Department of Medicine II, Kansai Medical University
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- Zeiher Andreas M.
- Department of Cardiology, Internal Medicine III, University of Frankfurt
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- Dimmeler Stefanie
- Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, University of Frankfurt
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- Shiojima Ichiro
- Department of Medicine II, Kansai Medical University
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Abstract
<p>Background:We previously identified circulating mesoangioblasts (cMABs), a subset of mesenchymal stem cells that express cardiac mesodermal markers, in patients undergoing cardiac surgery with cardiopulmonary bypass (CPB). We also found that hepatocyte growth factor (HGF) is upregulated during cardiac surgery with CPB in humans, and induces MAB-like cell mobilization in rodents. These results strongly suggest that heparin induced MAB mobilization via HGF upregulation. Here, we tested this hypothesis in patients undergoing cardiac surgery or cardiac catheterization. We also examined whether human cMABs are derived from the heart.</p><p>Methods and Results:Plasma HGF levels were determined by ELISA. Mononuclear cells isolated from blood samples were cultured on fibronectin-coated dishes, and outgrowing cMAB colonies were counted. We first confirmed that HGF upregulation and cMAB mobilization were observed before the start of CPB, excluding the possibility that CPB is the primary inducer of cMAB mobilization. We then examined patients undergoing cardiac catheterization and found that heparin significantly increased plasma HGF levels and the number of cMAB colonies in a dose-dependent manner. The results of simultaneous blood sampling from the aortic sinus, coronary sinus, and right atrium were consistent with the notion that human cMABs are derived from the heart.</p><p>Conclusions:Human cMABs are mobilized by heparin injection during cardiac surgery or cardiac catheterization, presumably via HGF upregulation.</p>
Journal
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- Circulation Journal
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Circulation Journal 82 (5), 1459-1465, 2018
The Japanese Circulation Society
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Details 詳細情報について
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- CRID
- 1390282680084297856
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- NII Article ID
- 130006726003
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- NII Book ID
- AA11591968
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- ISSN
- 13474820
- 13469843
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- NDL BIB ID
- 028957351
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- PubMed
- 28931787
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed