Anticoagulant Peptide Obtained from Fibrinogen Degradation Products by Plasmin. I

  • TAKAKI Akira
    Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
  • ISHIGURO Masatsune
    Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
  • FUNATSU Masaru
    Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

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  • Isolation of the Peptide

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A decapeptide which had an inhibitory effect on blood coagulation was isolated from the degradation products of bovine fibrinogen by plasmin on BioGel P-10 column. The purification of the active peptide was carried out by ion-exchange chromatography using both Dowex 1×2 and Dowex 50×2 column. Amino acid composition of the active peptide was determined to be Ser 0.94, Glu 3.88, Pro 1.04, Ala 1.00, Leu 1.97 and Lys 1.03.

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