キシログルカンから得られた八糖に対する抗体の免疫化学的性質とその植物組織中のキシログルカンの組織化学的同定への応用
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- 三崎 旭
- Department of Food and Nutrition , Faculty of Human Life Science, Osaka City University
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- 曽根 良昭
- Department of Food and Nutrition , Faculty of Human Life Science, Osaka City University
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- 柴田 聡明
- Department of Food and Nutrition , Faculty of Human Life Science, Osaka City University
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- 永田 敦子
- Department of Food and Nutrition , Faculty of Human Life Science, Osaka City University
書誌事項
- タイトル別名
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- Immunochemical Characterization of Antibodies to the Xyloglucan-octasaccharide and Histochemical Recognition of Xyloglucan in Plant Tissues
抄録
An octasaccharide [XGO8, composed of n-xylose, D-galactose, and D-glucose (3: 1: 4) ] purified from the (1→4) β-D-glucanase-digest of xyloglucan of tamarind (Tamarindus indica L .) seed, was coupled with bovine serum albumin (BSA), and used as an immunogen. The polyclonal antibodies raised in rabbits interacted with XGO8-BSA conjugates and of other oligosaccharide fragments, and also with the original xyloglucan. Hapten inhibition studies using various oligosaccharides showed that the antibodies recognize β-D-galactosyl- (1→2) -α-D-xylosyl, and also α-D-xylosyl side chains attached to the β- (14) -n-glucosyl backbone chain in the xyloglucan. The antibodies were used for histological localization of the xyloglucan in plant tissues, by either the fluorescence or the peroxidase-labeled immunostaining method. By the latter method it was shown that, in the soybean cotyledon, the location of the xyloglucan is restricted to the primary cell-walls .
収録刊行物
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- 応用糖質科学
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応用糖質科学 42 (1), 37-44, 1995
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390282680148764672
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- NII論文ID
- 130004257438
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- COI
- 1:CAS:528:DyaK2MXltlyitb8%3D
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- ISSN
- 18844898
- 13403494
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可