キシログルカンから得られた八糖に対する抗体の免疫化学的性質とその植物組織中のキシログルカンの組織化学的同定への応用

DOI
  • 三崎 旭
    Department of Food and Nutrition , Faculty of Human Life Science, Osaka City University
  • 曽根 良昭
    Department of Food and Nutrition , Faculty of Human Life Science, Osaka City University
  • 柴田 聡明
    Department of Food and Nutrition , Faculty of Human Life Science, Osaka City University
  • 永田 敦子
    Department of Food and Nutrition , Faculty of Human Life Science, Osaka City University

書誌事項

タイトル別名
  • Immunochemical Characterization of Antibodies to the Xyloglucan-octasaccharide and Histochemical Recognition of Xyloglucan in Plant Tissues

抄録

An octasaccharide [XGO8, composed of n-xylose, D-galactose, and D-glucose (3: 1: 4) ] purified from the (1→4) β-D-glucanase-digest of xyloglucan of tamarind (Tamarindus indica L .) seed, was coupled with bovine serum albumin (BSA), and used as an immunogen. The polyclonal antibodies raised in rabbits interacted with XGO8-BSA conjugates and of other oligosaccharide fragments, and also with the original xyloglucan. Hapten inhibition studies using various oligosaccharides showed that the antibodies recognize β-D-galactosyl- (1→2) -α-D-xylosyl, and also α-D-xylosyl side chains attached to the β- (14) -n-glucosyl backbone chain in the xyloglucan. The antibodies were used for histological localization of the xyloglucan in plant tissues, by either the fluorescence or the peroxidase-labeled immunostaining method. By the latter method it was shown that, in the soybean cotyledon, the location of the xyloglucan is restricted to the primary cell-walls .

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282680148764672
  • NII論文ID
    130004257438
  • DOI
    10.11541/jag1994.42.37
  • COI
    1:CAS:528:DyaK2MXltlyitb8%3D
  • ISSN
    18844898
    13403494
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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