プレシオモナスによるマルトースホスホリラーゼおよびトレハロースホスホリラーゼの生産と応用
書誌事項
- タイトル別名
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- Production and Application of Maltose Phosphorylase and Trehalose Phosphorylase by a Strain of Plesiomonas
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説明
A strain of Plesiomonas capable of producing intracellular thermostable maltose phosphorylase (MP) and trehalose phosphorylase (TP), which were useful for the production of trehalose from maltose, was isolated from mud of a Japanese seashore. The isolate (SH-35) grew well among the ranges of pH and temperature at pH 6-9 and 15-45°C with optima at pH 7.0 and 37°C by shaking cultivation, though the maximum yield of these enzymes were obtained at pH 7.5 and 34°C for MP and pH 8.0 and 37°C for TP with medium containing maltose as a carbon source and the mixture of Polypepton-S, yeast extract and urea as nitrogen sources. Optimum pH and temperature for producing trehalose were pH 7-8 and 55-60°C in the presence of 10-40% (w/w) maltose and 5-50 mM inorganic phosphate, and about 60% (as dry basis) of maltose was converted into trehalose by the simultaneous action of both enzymes before and after extraction from cells under the best conditions. These microbial and enzymatic characteristics are consistent with the industrial production of trehalose from maltose.
収録刊行物
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- 応用糖質科学
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応用糖質科学 42 (1), 19-25, 1995
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390282680148765824
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- NII論文ID
- 130004257437
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- COI
- 1:CAS:528:DyaK2MXltlyitLc%3D
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- ISSN
- 18844898
- 13403494
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
