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- 大井 俊彦
- Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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- 藤本 啓明
- Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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- 王 三郎
- Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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- 滝沢 登志雄
- Bioscience Laboratory, Meiji Seika Kaisha Ltd.
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- 日高 秀昌
- Bioscience Laboratory, Meiji Seika Kaisha Ltd.
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- 小倉 セイ
- Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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- 村尾 澤夫
- Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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- 荒井 基夫
- Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
書誌事項
- タイトル別名
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- Purification and Some Properties of Aspergillus aculeatus β-Xylosidase
- Aspergillus aculeatus ユライ ベータ キシロシダーゼ ノ
この論文をさがす
抄録
Extracellular β-xylosidase ([EC 3.2.1.37]: xylan 1, 4-β-xylosidase) was purified from a culture filtrate of Aspergillus aculeatus No. F-50 by column chromatography using DEAE-Sephadex A-50, Sephacry 5-200, and DEAE-Toyopearl 650M columns, and preparative isoelectric focusing. The purified enzyme was homogeneous on SDS-polyacrylamide gel electrophoresis . The molecular weight was about 105, 000 by SDS-PAGE and its p1 value was 4.3. The optimum pH and temperature for the /3 -xylosidase activities were 2.0 and 70°C, respectively. The β-xylosidase was stable for 30 min at 50°C and stable between pH 3 and 7. The enzyme activity was strongly inhibited by Cu2+, Mn2+, and Hg2+. The enzyme hydrolyzed xylooligosaccharides, xylobiose through xylopentaose, to form xylose. The β-xylosidase showed potent activity towards larchwood xylan .
収録刊行物
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- 応用糖質科学
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応用糖質科学 42 (1), 45-48, 1995
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390282680148776704
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- NII論文ID
- 10008253971
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- NII書誌ID
- AN10453916
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- COI
- 1:CAS:528:DyaK2MXltlyitbw%3D
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- ISSN
- 18844898
- 13403494
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- NDL書誌ID
- 3616893
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可