Caveolae-Independent Activation of Protein Kinase A in Rat Neonatal Myocytes
-
- Fukutomi Masashi
- Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University
-
- Nishida Motohiro
- Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University
-
- Maruyama Yoshiko
- Laboratory of Cellular Signaling, Graduate School of Pharmaceutical Sciences, University of Tokyo
-
- Kobayashi Hiroyuki
- Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University
-
- Kurose Hitoshi
- Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University
Search this article
Abstract
Cardiomyocytes express both β1- and β2-adrenergic receptors, and these receptors play a differential role in chronotropic and inotropic effects of the heart. Caveolae are known as an important regulator of G-protein-coupled receptor signaling. In the present report, we examined whether caveolae have a role in β-adrenergic receptor-stimulated cAMP production and protein kinase A activation in neonatal myocytes. Isoproterenol-stimulated cAMP production was mediated by β1- and β2-subtypes, which depends on the receptor number of each subtype. However, protein kinase A activation was exclusively mediated by the β1-subtype. Disruption of caveolae by methyl-β-cyclodextrin treatment did not affect the relative contribution of subtypes to isoproterenol-stimulated cAMP production. β1-Subtype-mediated protein kinase A activation was also not affected by the disruption of caveolae. These results suggest that β1-adrenergic receptor-mediated protein kinase A activation is compartmentalized and independent of caveolae.<br>
Journal
-
- Journal of Pharmacological Sciences
-
Journal of Pharmacological Sciences 98 (2), 168-174, 2005
The Japanese Pharmacological Society
- Tweet
Details 詳細情報について
-
- CRID
- 1390282680152452096
-
- NII Article ID
- 10025728483
-
- NII Book ID
- AA11806667
-
- ISSN
- 13478648
- 13478613
-
- NDL BIB ID
- 7337896
-
- PubMed
- 15942126
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed