Imaging and Functional Analysis of Blood Flow in Organic Microcirculation
-
- Minamitani Haruyuki
- Institute of Biomedical Engineering, Graduate School of Science and Technology, Keio University
-
- Tsukada Kosuke
- Department of Physiology, Kawasaki Medical School
-
- Sekizuka Eiichi
- National Saitama Hospital
-
- Oshio Chikara
- Oshio Clinic
書誌事項
- タイトル別名
-
- Optical Bioimaging: From Living Tissue to a Single Molecule: Imaging and Functional Analysis of Blood Flow in Organic Microcirculation
この論文をさがす
抄録
Activity of blood cells, erythrocytes, leucocytes, and platelets, in microcirculation was observed by using an intravital microscope and confocal laser scanning microscope connected with an image processing system including fluorescence and phosphorescence emission methods. Dynamic functions of the blood flow were mainly observed in mesentery, brain, and liver tissues of rats. The results are summarized as follows: Deformability of diabetic erythrocytes was significantly lower than that of healthy controls, particularly at high shear rate. The spring constant and Young’s modulus of diabetic erythrocytes obviously stiffened, making them hard to deform in the capillary. During hemorrhagic shock and thrombosis, flow velocity and oxygen partial pressure of blood decreased in the brain and liver tissues that can be visualized by using FITC stained erythrocytes and Pd-porphyrin derivative as a pO2 probe. Platelet adhesion and thrombus formation in the micro-vessels accelerated under the photodynamic reaction; diabetic platelets showed augmented adhesion and aggregation on the vessel wall which was followed by acute thromboembolism. Active oxygen radicals take part in thrombus formation, accompanied with adhesion of the activated leucocytes. Fluorescent dye probes, rhodamine G and acridine orange, are quite useful for visualization of the flow behavior of platelets and leucocytes, respectively.<br>
収録刊行物
-
- Journal of Pharmacological Sciences
-
Journal of Pharmacological Sciences 93 (3), 227-233, 2003
公益社団法人 日本薬理学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282680153188352
-
- NII論文ID
- 10012544759
-
- NII書誌ID
- AA11806667
-
- ISSN
- 13478648
- 13478613
-
- NDL書誌ID
- 6757706
-
- PubMed
- 14646237
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可