Screening for Control Genes in Mouse Hippocampus After Transient Forebrain Ischemia Using High-Density Oligonucleotide Array

DOI Web Site 被引用文献1件 参考文献35件
  • Nishida Yayoi
    Division of Genomic Epidemiology and Clinical Trials, Nihon University School of Medicine, Japan
  • Sugahara-Kobayashi Megumi
    Department of Pharmacology, Nihon University School of Medicine, Japan
  • Takahashi Yasuo
    Division of Genomic Epidemiology and Clinical Trials, Nihon University School of Medicine, Japan
  • Nagata Toshihito
    Department of Cell Regeneration and Transplantation, Nihon University School of Medicine, Japan
  • Ishikawa Koichi
    Department of Pharmacology, Nihon University School of Medicine, Japan
  • Asai Satoshi
    Division of Genomic Epidemiology and Clinical Trials, Nihon University School of Medicine, Japan

この論文をさがす

抄録

In conventional relative gene expression analysis (Northern blotting, RT-PCR, and in situ hybridization), housekeeping genes such as the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin genes, whose expression levels are considered stable, have been used as control genes for normalization of RNA quantitation. However, it has been reported that the expression levels of these two control genes are affected by ischemia. Therefore, we have been searching for novel control genes whose expression levels are stable in a mouse model of transient forebrain ischemia. Using the GeneChip Mu6500 array set, we monitored the expression levels of approximately 6000 murine genes in the mouse hippocampus during 24 h of ischemia-reperfusion. To select stable genes, we applied the restricted criterion of a 1.5-fold change in expression level as the threshold. By adding statistical analysis with this criterion, we identified 10 genes as candidates for control genes from the GeneChip data. In this criterion, GAPDH and β-actin genes were not included in the 10 genes as candidates for control genes. The present findings might be relevant to the use of control genes in quantitation of RNA, particularly in the study of mouse transient forebrain ischemia.<br>

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (35)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ