The Site Where Newly Synthesized ATP Is Necessary for Tension Development in α-Toxin Permeabilized Preparations of Rat Proximal Colon
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- Takeuchi Tadayoshi
- Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Science, Osaka Prefecture University
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- Fujita Akikazu
- Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Science, Osaka Prefecture University
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- Kushida Masahiko
- Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Science, Osaka Prefecture University
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- Hata Fumiaki
- Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Science, Osaka Prefecture University
書誌事項
- タイトル別名
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- The Site Where Newly Synthesized ATP Is Necessary for Tension Development in .ALPHA.-Toxin Permeabilized Preparations of Rat Proximal Colon
- Site Where Newly Synthesized ATP Is Necessary for Tension Development in アルファ Toxin Permeabilized Preparations of Rat Proximal Colon
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抄録
Since it was suggested in our previous study that ATP newly synthesized from ADP and phosphocreatine (PCr) by creatine kinase had an important role in Ca2+-induced phasic contraction in α-toxin permeabilized smooth muscle of rat proximal colon, we studied the role of newly synthesized ATP on myosin ATPase activity, by assessing a rate of force development as an index of myosin ATPase activity. The α-toxin-permeabilized preparations were thiophosphorylated by treatment with ATPγS. After the thiophosphorylation, the contraction induced by ATP plus PCr in the absence of Ca2+ reached the maximum at 30 s. When PCr was omitted from the bathing solution, the initial rate of the contraction was significantly slower, while the level of myosin light chain thiophosphorylation remained unchanged. An inhibitor of creatine kinase slowed the initial contractile rate to a rate similar to that induced by ATP alone. ADPβS had no effect on ATP plus PCr-induced contraction, suggesting that accumulation of ADP does not affect the initial rate of the contraction. PCr alone did not contract the thiophosphorylated-preparations. However, in the presence of ADP, PCr induced contraction at the initial rate which was slower than that induced by ATP plus PCr. These results indicate that newly synthesized ATP together with preexisting ATP is utilized as a substrate for myosin ATPase.<br>
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 91 (4), 277-284, 2003
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390282680154364800
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- NII論文ID
- 130000073686
- 30003472502
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- NII書誌ID
- AA11806667
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- COI
- 1:CAS:528:DC%2BD3sXjtlOqtrw%3D
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 6510749
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- PubMed
- 12719656
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可