Down-Regulation of Myosin Light Chain Kinase Expression in Vascular Smooth Muscle Cells Accelerates Cell Proliferation: Requirement of Its Actin-binding Domain for Reversion to Normal Rates
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- Wang Hong Hui
- Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan
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- Nakamura Akio
- Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan
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- Yoshiyama Shinji
- Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan
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- Ishikawa Ryoki
- Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan
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- Cai Na
- Department of Biochemistry, College of Life Sciences, Nankai University, P.R. China Department of Biochemistry, College of Life Sciences, Nankai University, P.R. China
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- Ye Li-Hong
- Department of Biochemistry, College of Life Sciences, Nankai University, P.R. China Department of Biochemistry, College of Life Sciences, Nankai University, P.R. China
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- Takano-Ohmuro Hiromi
- Research Institute of Pharmaceutical Sciences, Musashino University, Japan Research Institute of Pharmaceutical Sciences, Musashino University, Japan
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- Kohama Kazuhiro
- Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan Research Institute of Pharmaceutical Sciences, Musashino University, Japan Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan Research Institute of Pharmaceutical Sciences, Musashino University, Japan
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抄録
Myosin light-chain kinase (MLCK) is a multi-domain protein with kinase and actin-binding domains, among others. Deficiency of MLCK expression in GBaSM-4 vascular smooth muscle cells enhanced cell proliferation rate and shortened cell doubling time. Transient transfection of the MLCK-deficient cells with cDNA constructs of either wild-type MLCK or its mutant lacking the kinase activity reverted the cell proliferation rate to that of wild-type cells, whereas that of MLCK lacking the actin-binding domain maintained cell proliferation at an elevated rate similar to the MLCK-deficient cells. Thus, the actin-binding domain of MLCK seems to play a role in regulating cell proliferation.
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 119 (1), 91-96, 2012
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390282680155027968
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- NII論文ID
- 10030760488
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- NII書誌ID
- AA11806667
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- COI
- 1:CAS:528:DC%2BC38XnvVKnsL8%3D
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 023634756
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- PubMed
- 22510518
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可