Protective Effect of Demethylation Treatment on Cigarette Smoke Extract–Induced Mouse Emphysema Model
-
- Zhang Hongliang
- Emergency Department, Second Xiangya Hospital, Central South University, China
-
- Chen Ping
- Pulmonary Department, Second Xiangya Hospital, Central South University, China
-
- Zeng Huihui
- Pulmonary Department, Second Xiangya Hospital, Central South University, China
-
- Zhang Yan
- Pulmonary Department, Second Xiangya Hospital, Central South University, China
-
- Peng Hong
- Pulmonary Department, Second Xiangya Hospital, Central South University, China
-
- Chen Yan
- Pulmonary Department, Second Xiangya Hospital, Central South University, China
-
- He Zhihui
- Pulmonary Department, Second Xiangya Hospital, Central South University, China
この論文をさがす
抄録
In the present study, we explored the effects of demethylation in a cigarette smoke extract (CSE)-induced mouse emphysema model. Animals were randomly assigned to the control group, CSE group, 5-aza-2′-deoxycytidine (AZA) group, and CSE+AZA group (n = 10 per group). The mitochondrial transcription factor A (mtTFA) promoter methylation increased over 4-fold in the CSE group compared with the control group, which was reversed by AZA. The mtTFA and the cytochrome c oxidase subunit II (COX II) mRNA and protein levels were decreased approximately 3-fold in the CSE group compared with the control group, which was largely restored by AZA. Histological analysis showed that the CSE group exhibited emphysema compared with the control, which was alleviated by AZA. In addition, CSE significantly induced lung cell apoptosis and decreased lung function and lung mitochondrial COX activity, which was mostly restored by AZA. In conclusion, we for the first time provide evidence that demethylation therapy with AZA can effectively improve emphysema, lung function, lung cell apoptosis, and lung mitochondrial COX activity in a CSE-induced mouse emphysema model, which adds fresh insight into the therapeutic potential of demethylating agents in the prevention and treatment of cigarette smoke-induced emphysema.
収録刊行物
-
- Journal of Pharmacological Sciences
-
Journal of Pharmacological Sciences 123 (2), 159-166, 2013
公益社団法人 日本薬理学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282680156615680
-
- NII論文ID
- 130003382587
-
- NII書誌ID
- AA11806667
-
- COI
- 1:CAS:528:DC%2BC3sXhslertrrK
-
- ISSN
- 13478648
- 13478613
-
- NDL書誌ID
- 024954861
-
- PubMed
- 24141367
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可